RGD Reference Report - Utilization of marker-assisted congenics to map two blood pressure quantitative trait loci in Dahl rats. - Rat Genome Database

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Utilization of marker-assisted congenics to map two blood pressure quantitative trait loci in Dahl rats.

Authors: Deng, AY  Dutil, J  Sivo, Z 
Citation: Deng AY, etal., Mamm Genome 2001 Aug;12(8):612-6.
RGD ID: 69734
Pubmed: (View Article at PubMed) PMID:11471055

A broad Chromosome (Chr) 10 region of the Dahl salt-sensitive (S) rat was shown by linkage and the use of congenic strains to contain a blood pressure (BP) quantitative trait locus (QTL). To further narrow down the region harboring the QTL, four congenic strains carrying smaller segments were made by replacing various segments of the S rats with the homologous segments of the Lewis (LEW) rats. The construction of these congenic strains was facilitated by a genome-wide marker screening. One congenic strain, assigned as S.L4, showed a BP-lowering effect, and the region harboring a BP QTL, designated QTL1, is localized to a segment of about 15 cM. Two other strains, assigned as S.L2 and S.L5, contained an overlapping segment, and both showed a BP-lowering effect. In contrast, the fourth congenic strain, assigned as S.L1, contained a smaller and shared fragment with S.L2 and S.L5, but it did not have a BP-lowering effect. Deducing from the segment in common in S.L2 and S.L5, and not shared between S.L1 and both congenic strains S.L2 and S.L5, the region harboring a QTL, designated as QTL2, was narrowed to about 12 cM. The current work showed the general applicability of the 'speed congenic' approach to map and fine-map BP QTL.



Disease Annotations    
Hypotension  (IDA)

Phenotype Annotations    

Mammalian Phenotype
Objects Annotated

QTLs
Bp71  (Blood pressure QTL 71)
Bp72  (Blood pressure QTL 72)

Objects referenced in this article
Strain SS.LEW-(D10Mco1-D10Mco31)/Ayd null Rattus norvegicus
Strain SS.LEW-(D10Rat11-D10Mgh1)/Ayd null Rattus norvegicus
Strain SS.LEW-(D10Rat141-D10Mgh1)/Ayd null Rattus norvegicus

Additional Information