RGD Reference Report - Genetic linkage analysis of X-ray hypersensitivity in the LEC mutant rat. - Rat Genome Database

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Genetic linkage analysis of X-ray hypersensitivity in the LEC mutant rat.

Authors: Agui, T  Miyamoto, T  Jung, CG  Tsumagari, T  Masuda, K  Manabe, T 
Citation: Agui T, etal., Mamm Genome 2000 Oct;11(10):862-5.
RGD ID: 69702
Pubmed: PMID:11003700   (View Abstract at PubMed)

The LEC rat has been reported to exhibit X-ray hypersensitivity and deficiency in DNA double-strand break (DSB) repair. The present study was performed to map the locus responsible for this phenotype, the xhs (X-ray hypersensitivity), as the first step in identifying the responsible gene. Analysis of the progeny of (BN x LEC)F(1) x LEC backcrosses indicated that the X-ray hypersensitive phenotype was controlled by multiple genetic loci in contrast to the results reported previously. Quantitative trait loci (QTL) linkage analysis revealed two responsible loci located on Chromosomes (Chr) 4 and 1. QTL on Chr 4 exhibited very strong linkage to the X-ray hypersensitive phenotype, while QTL on Chr 1 showed weak linkage. The Rad52 locus, mutation of which results in hypersensitivity to ionizing radiation and impairment of DNA DSB repair in yeast, was reported to be located on the synteneic regions of mouse Chr 6 and human Chr 12. However, mapping of the rat Rad52 locus indicated that it was located 23 cM distal to the QTL on Chr 4. Furthermore, none of the radio-sensitivity-related loci mapped previously in the rat chromosome were identical to the QTL on Chrs 4 and 1 in the LEC rat. Thus, it seems that X-ray hypersensitivity in the LEC rat is caused by mutation(s) in as-yet-undefined genes.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Experimental Radiation Injuries  IDA 69702; 69702 RGD 

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
decreased mortality induced by ionizing radiation  IAGP 69702compared to LEC/NcuRGD 
increased cellular sensitivity to X-ray irradiation  QTM 69702; 69702 RGD 
increased mortality induced by ionizing radiation  IAGP 69702compared to BN/SeaRGD 
Objects Annotated

Xhs1  (X-ray hypersensitivity QTL 1)
Xhs2  (X-ray hypersensitivity QTL 2)

BN/Sea  (BN/Sea)
LEC/Ncu  (NA)

Objects referenced in this article
Strain BN.LEC-(D4Rat128-D4Rat106)/Hkv null Rattus norvegicus
Strain BN.LEC-(D4Rat128-D4Rat238)/Hkv null Rattus norvegicus
Strain BN.LEC-(D4Rat184-D4Rat238)/Hkv null Rattus norvegicus
Strain LEC.BN-(D4Mgh16-D4Rat233)(D4Rat271-D4Rat238)/Hkv null Rattus norvegicus
Strain LEC.BN-(D4Mgh16-D4Rat233)/Hkv null Rattus norvegicus

Additional Information