RGD Reference Report - Interaction of protein kinase C zeta with ZIP, a novel protein kinase C-binding protein. - Rat Genome Database

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Interaction of protein kinase C zeta with ZIP, a novel protein kinase C-binding protein.

Authors: Puls, A  Schmidt, S  Grawe, F  Stabel, S 
Citation: Puls A, etal., Proc Natl Acad Sci U S A 1997 Jun 10;94(12):6191-6.
RGD ID: 68823
Pubmed: PMID:9177193   (View Abstract at PubMed)
PMCID: PMC21025   (View Article at PubMed Central)

The atypical protein kinase C (PKC) member PKC-zeta has been implicated in several signal transduction pathways regulating differentiation, proliferation or apoptosis of mammalian cells. We report here the identification of a cytoplasmic and membrane-associated protein that we name zeta-interacting protein (ZIP) and that interacts with the regulatory domain of PKC-zeta but not classic PKCs. The structural motifs in ZIP include a recently defined ZZ zinc finger as a potential protein binding module, two PEST sequences and a novel putative protein binding motif with the consensus sequence YXDEDX5SDEE/D. ZIP binds to the pseudosubstrate region in the regulatory domain of PKC-zeta and is phosphorylated by PKC-zeta in vitro. ZIP dimerizes via the same region that promotes binding to PKC-zeta suggesting a competitive situation between ZIP:ZIP and ZIP:PKC-zeta complexes. In the absence of PKC-zeta proper subcellular localization of ZIP is impaired and we show that intracellular targeting of ZIP is dependent on a balanced interaction with PKC-zeta. Taking into account the recent isolation of ZIP by others in different contexts we propose that ZIP may function as a scaffold protein linking PKC-zeta to protein tyrosine kinases and cytokine receptors.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Nup62Ratprotein binding enablesIPIUniProtKB:O08623PMID:9177193UniProt 
Sqstm1Ratprotein binding enablesIPIUniProtKB:P17955PMID:9177193UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Nup62  (nucleoporin 62)
Sqstm1  (sequestosome 1)


Additional Information