RGD Reference Report - Myelin-associated oligodendrocytic basic protein mRNAs reside at different subcellular locations. - Rat Genome Database

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Myelin-associated oligodendrocytic basic protein mRNAs reside at different subcellular locations.

Authors: Gould, RM  Freund, CM  Barbarese, E 
Citation: Gould RM, etal., J Neurochem 1999 Nov;73(5):1913-24.
RGD ID: 633193
Pubmed: PMID:10537049   (View Abstract at PubMed)

The mRNAs for two myelin proteins, myelin basic protein (MBP) and myelin-associated oligodendrocytic basic protein (MOBP)-81A, are uniquely located at sites where myelin sheaths are assembled. Here, we use subcellular fractionation to show that four MOBP mRNAs, like MBP mRNA, are located at sites of myelin sheath assembly, and that three other MOBP mRNAs are located in oligodendrocyte soma. The MOBP-81 protein is found in myelin and in another subcellular fraction, whereas other myelin proteins, including MBP, 2',3'-cyclic nucleotide 3'-phosphodiesterase, and myelin-associated glycoprotein, are largely restricted to myelin. Different MBP mRNAs are generated by alternative splicing. All of them contain an RNA transport sequence (RTS) that directs them to sites in oligodendrocytes, where myelin sheaths are assembled. Consequently, all are enriched in myelin. After fractionation, four MOBP mRNAs, MOBP-71, MOBP-81A, MOBP-99, and MOBP-169 (identified in this study), are enriched in myelin. These mRNAs contain a common exon, exon 8b, which has a nucleotide sequence that is similar to MBP mRNA RTS. This sequence likely directs these mRNAs to sites of myelin sheath assembly. Three other MOBP mRNAs, MOBP-69, MOBP-81B, and MOBP-170, lack this exon. Their subcellular distribution indicates that they are largely retained in oligodendrocyte soma. We conclude that the distribution of MOBPs in oligodendrocytes is strongly influenced by alternative splicing of the corresponding mRNAs.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MbpRatcompact myelin  IDA MMO:0000679RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mbp  (myelin basic protein)

Objects referenced in this article
Gene Mobp myelin-associated oligodendrocyte basic protein Rattus norvegicus

Additional Information