RGD Reference Report - The native rat olfactory cyclic nucleotide-gated channel is composed of three distinct subunits. - Rat Genome Database

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The native rat olfactory cyclic nucleotide-gated channel is composed of three distinct subunits.

Authors: Bonigk, W  Bradley, J  Muller, F  Sesti, F  Boekhoff, I  Ronnett, GV  Kaupp, UB  Frings, S 
Citation: Bonigk W, etal., J Neurosci 1999 Jul 1;19(13):5332-47.
RGD ID: 632392
Pubmed: PMID:10377344   (View Abstract at PubMed)
PMCID: PMC6782342   (View Article at PubMed Central)

Cyclic nucleotide-gated (CNG) channels play central roles in visual and olfactory signal transduction. In the retina, rod photoreceptors express the subunits CNCalpha1 and CNCbeta1a. In cone photoreceptors, only CNCalpha2 expression has been demonstrated so far. Rat olfactory sensory neurons (OSNs) express two homologous subunits, here designated CNCalpha3 and CNCalpha4. This paper describes the characterization of CNCbeta1b, a third subunit expressed in OSNs and establishes it as a component of the native channel. CNCbeta1b is an alternate splice form of the rod photoreceptor CNCbeta1a subunit. Analysis of mRNA and protein expression together suggest co-expression of all three subunits in sensory cilia of OSNs. From single-channel analyses of native rat olfactory channels and of channels expressed heterologously from all possible combinations of the CNCalpha3, -alpha4, and -beta1b subunits, we conclude that the native CNG channel in OSNs is composed of all three subunits. Thus, CNG channels in both rod photoreceptors and olfactory sensory neurons result from coassembly of specific alpha subunits with various forms of an alternatively spliced beta subunit.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Cnga2Ratpotassium ion transport involved_inIDA PMID:10377344UniProt 
Cnga4Ratpotassium ion transport involved_inIDA PMID:10377344UniProt 
Cngb1Ratpotassium ion transport involved_inIDA PMID:10377344UniProt 
Cnga2Ratsodium ion transport involved_inIDA PMID:10377344UniProt 
Cnga4Ratsodium ion transport involved_inIDA PMID:10377344UniProt 
Cngb1Ratsodium ion transport involved_inIDA PMID:10377344UniProt 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Cnga2Ratnon-motile cilium membrane located_inIDA PMID:10377344UniProt 
Cnga4Ratnon-motile cilium membrane located_inIDA PMID:10377344UniProt 
Cngb1Ratnon-motile cilium membrane located_inIDA PMID:10377344UniProt 

Molecular Function

  

Objects Annotated

Genes (Rattus norvegicus)
Cnga2  (cyclic nucleotide gated channel subunit alpha 2)
Cnga4  (cyclic nucleotide gated channel subunit alpha 4)
Cngb1  (cyclic nucleotide gated channel subunit beta 1)


Additional Information