RGD Reference Report - Gamma-synergin: an EH domain-containing protein that interacts with gamma-adaptin. - Rat Genome Database

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Gamma-synergin: an EH domain-containing protein that interacts with gamma-adaptin.

Authors: Page, LJ  Sowerby, PJ  Lui, WW  Robinson, MS 
Citation: Page LJ, etal., J Cell Biol 1999 Sep 6;146(5):993-1004.
RGD ID: 631945
Pubmed: PMID:10477754   (View Abstract at PubMed)
PMCID: PMC2169493   (View Article at PubMed Central)

The AP-1 adaptor complex is associated with the TGN, where it links selected membrane proteins to the clathrin lattice, enabling these proteins to be concentrated in clathrin-coated vesicles. To identify other proteins that participate in the clathrin-coated vesicle cycle at the TGN, we have carried out a yeast two- hybrid library screen using the gamma-adaptin subunit of the AP-1 complex as bait. Two novel, ubiquitously expressed proteins were found: p34, which interacts with both gamma-adaptin and alpha-adaptin, and gamma-synergin, an alternatively spliced protein with an apparent molecular mass of approximately 110-190 kD, which only interacts with gamma-adaptin. gamma-Synergin is associated with AP-1 both in the cytosol and on TGN membranes, and it is strongly enriched in clathrin-coated vesicles. It binds directly to the ear domain of gamma-adaptin and it contains an Eps15 homology (EH) domain, although the EH domain is not part of the gamma-adaptin binding site. In cells expressing alpha-adaptin with the gamma-adaptin ear, a construct that goes mainly to the plasma membrane, much of the gamma-synergin is also rerouted to the plasma membrane, indicating that it follows AP-1 onto membranes rather than leading it there. The presence of an EH domain suggests that gamma-synergin links the AP-1 complex to another protein or proteins.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SynrgRatclathrin coat of trans-Golgi network vesicle  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Synrg  (synergin, gamma)

Objects referenced in this article
Gene SYNRG synergin gamma Homo sapiens
Gene Ap1g1 adaptor protein complex AP-1, gamma 1 subunit Mus musculus
Gene Aagab alpha- and gamma-adaptin binding protein Rattus norvegicus
Gene Ap1g1 adaptor related protein complex 1 subunit gamma 1 Rattus norvegicus
Gene Ap1s1 adaptor related protein complex 1 subunit sigma 1 Rattus norvegicus
Gene Ap1s2 adaptor related protein complex 1 subunit sigma 2 Rattus norvegicus
Gene Ap2a2 adaptor related protein complex 2 subunit alpha 2 Rattus norvegicus

Additional Information