RGD Reference Report - Mapping of QTL influencing saccharin consumption in the selectively bred alcohol-preferring and -nonpreferring rat lines. - Rat Genome Database

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Mapping of QTL influencing saccharin consumption in the selectively bred alcohol-preferring and -nonpreferring rat lines.

Authors: Foroud, T  Bice, P  Castelluccio, P  Bo, R  Ritchotte, A  Stewart, R  Lumeng, L  Li, TK  Carr, L 
Citation: Foroud T, etal., Behav Genet 2002 Jan;32(1):57-67.
RGD ID: 631229
Pubmed: PMID:11958543   (View Abstract at PubMed)

The inbred preferring (iP) and nonpreferring (iNP) rat strains were derived from the selectively bred alcohol-preferring (P) and alcohol-nonpreferring (NP) lines. Previously, 381 iP x iNP F2 progeny were generated to identify quantitative trait loci (QTLs) influencing alcohol consumption and preference. Saccharin consumption (ml/48 h) and saccharin intake (ml/kg/day) were also measured in the F2 sample and were significantly correlated with both alcohol consumption and preference (all r > or = .20, p < .0001), suggesting that there might be some QTLs influencing both saccharin and alcohol phenotypes. We have performed a genome screen using F2 animals with extreme saccharin or alcohol consumption to identify QTLs contributing to saccharin-related phenotypes. Lod scores greater than 2.0 were found on chromosomes 3, 16 and 18 in this sample. Additional genotyping was performed in these regions in the full sample of 381 F2 progeny to further characterize these putative QTLs. On chromosome 3, the maximum lod score in the full sample was 2.7 with saccharin consumption. This QTL appears to overlap with a QTL identified for alcohol consumption in the iP and iNP lines and has pleiotropic effects on both phenotypes. Interestingly, this region of rat chromosome 3 is syntenic with mouse chromosome 2, where a QTL influencing alcohol preference has been previously reported. The QTL on chromosome 16 has a maximum lod score of 4.0 with saccharin intake and 2.6 with saccharin consumption. The QTL on chromosome 18 has a maximum lod score of 2.7 with saccharin consumption. Taken together, these data provide the first results of a genome screen for QTLs contributing to saccharin phenotypes in the rat.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
alcohol use disorder  IDA 631229 RGD 
alcohol use disorder  IAGP 631229 RGD 

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
alcohol preference  QTM 631229 RGD 
saccharin preference  IAGP 631229; 631229; 631229 RGD 
saccharin preference  IDA 631229; 631229; 631229 RGD 
Objects Annotated

QTLs
Alc8  (Alcohol consumption QTL 8)
Sach1  (Saccharin preference QTL 1)
Sach2  (Saccharin preference QTL 2)
Sach3  (Saccharin preference QTL 3)

Strains
iNP/Iusm  (alcohol-nonpreferring)
iP/Iusm  (inbred alcohol-preferring)


Additional Information