RGD Reference Report - Different functions of QTL for estrogen-dependent tumor growth of the rat pituitary. - Rat Genome Database

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Different functions of QTL for estrogen-dependent tumor growth of the rat pituitary.

Authors: Wendell, DL  Daun, SB  Stratton, MB  Gorski, J 
Citation: Wendell DL, etal., Mamm Genome 2000 Oct;11(10):855-61.
RGD ID: 629550
Pubmed: PMID:11003699   (View Abstract at PubMed)

Estrogen treatment to rats of the Fischer 344 (F344) strain induces growth of pituitary tumors that exhibit accelerated cell proliferation, breakdown of basement membrane, and formation of hemorrhagic lakes. Estrogen-dependent pituitary growth is due to variation in a group of quantitative trait loci (QTL), called Edpm for estrogen-dependent pituitary mass, that we previously identified in an F(2) intercross of F344 and the tumor-resistant Brown Norway strain. We previously identified 5 QTL, and microsatellite markers developed since our earlier work have allowed us to scan new chromosomal regions, resulting in two new QTL for estrogen-dependent pituitary mass: Edpm9-2 and a possible QTL on the X Chromosome (Chr). Here we report evidence that these QTL differ from each other in how they affect growth. To examine the effect of the Edpm QTL on biochemical components of tumor growth, we tested their effects in 138 progeny of a backcross to the F344 strain which were given a 10-week chronic estrogen treatment. Hemoglobin/DNA ratio (a measure of blood volume relative to cell number) and total pituitary DNA (a measure of cell number) correlated only weakly, and very large pituitaries were observed which had a low hemoglobin/DNA ratio resembling a normal gland. Through QTL mapping, we found that Edpm2-1, Edpm3, Edpm5, and Edpm9-2 all had significant effects on pituitary mass, but Edpm2-1 and Edpm9-2 primarily affected DNA content, Edpm5 primarily affected hemoglobin/DNA ratio, and Edpm3 affected all traits equally.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Pituitary Neoplasms  IAGP 629550; 629550 RGD 

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
impaired basement membrane formation  QTM 629550 RGD 
pituitary gland hyperplasia  QTM 629550; 629550 RGD 
Objects Annotated

QTLs
Edpm11  (Estrogen-dependent pituitary mass QTL 11)
Edpm12  (Estrogen-dependent pituitary mass QTL 12)

Strains
BN/SsNHsd  (NA)
F344/NHsd  (F344/NHsd)


Additional Information