RGD Reference Report - The mammalian immediate-early TIS21 protein and the leukemia-associated BTG1 protein interact with a protein-arginine N-methyltransferase. - Rat Genome Database

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The mammalian immediate-early TIS21 protein and the leukemia-associated BTG1 protein interact with a protein-arginine N-methyltransferase.

Authors: Lin, WJ  Gary, JD  Yang, MC  Clarke, S  Herschman, HR 
Citation: Lin WJ, etal., J Biol Chem 1996 Jun 21;271(25):15034-44.
RGD ID: 61630
Pubmed: PMID:8663146   (View Abstract at PubMed)

The TIS21 immediate-early gene and leukemia-associated BTG1 gene encode proteins with similar sequences. Two-hybrid analysis identified a protein that interacts with TIS21 and BTG1. Sequence motifs associated with S-adenosyl-L-methionine binding suggested this protein might have methyltransferase activity. A glutathione S-transferase (GST) fusion of the putative methyltransferase modifies arginine residues, in appropriate protein substrates, to form NG-monomethyl and NG,NG-dimethylarginine (asymmetric). We term the protein- arginine N-methyltransferase (EC 2.1.1.23) gene "PRMT1, " for protein-arginine methyltransferase 1. GST-TIS21 and GST-BTG1 fusion proteins qualitatively and quantitatively modulate endogenous PRMT1 activity, using control and hypomethylated RAT1 cell extracts as methyl-accepting substrates. PRMT1 message appears ubiquitous, and is constitutive in mitogen-stimulated cells. Modulation of PRMT1 activity by transiently expressed regulatory subunits may be an additional mode of signal transduction following ligand stimulation.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Prmt1Ratpeptidyl-arginine methylation  IDA  RGD 
Prmt1Ratpeptidyl-arginine methylation, to asymmetrical-dimethyl arginine acts_upstream_of_or_withinIDA PMID:8663146MGI 
Prmt1Ratpeptidyl-arginine methylation, to asymmetrical-dimethyl arginine  IDA  RGD 
Prmt1Ratpeptidyl-arginine omega-N-methylation acts_upstream_of_or_withinIDA PMID:8663146MGI 

Molecular Function

  

Objects Annotated

Genes (Rattus norvegicus)
Prmt1  (protein arginine methyltransferase 1)

Objects referenced in this article
Gene Btg1 BTG anti-proliferation factor 1 Mus musculus
Gene Btg2 BTG anti-proliferation factor 2 Mus musculus
Gene Prmt1 protein arginine N-methyltransferase 1 Mus musculus

Additional Information