RGD Reference Report - Butylated hydroxyanisole alters rat 5α-reductase and 3α-hydroxysteroid dehydrogenase: Implications for influences of neurosteroidogenesis. - Rat Genome Database

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Butylated hydroxyanisole alters rat 5α-reductase and 3α-hydroxysteroid dehydrogenase: Implications for influences of neurosteroidogenesis.

Authors: Guo, Jingjing  Li, Lili  Zhou, Songyi  Su, Ying  Li, Xiaoheng  Sun, Jianliang  Ge, Ren-Shan 
Citation: Guo J, etal., Neurosci Lett. 2017 Jul 13;653:132-138. doi: 10.1016/j.neulet.2017.05.034. Epub 2017 May 24.
RGD ID: 597621557
Pubmed: PMID:28552457   (View Abstract at PubMed)
DOI: DOI:10.1016/j.neulet.2017.05.034   (Journal Full-text)

Butylated hydroxyanisole is a synthetic antioxidant. It may affect the function of the nerve system. The objective of the present study is to investigate the direct effects of butylated hydroxyanisole on rat brain neurosteroidogenic 5α-reductase 1 (SRD5A1), 3α-hydroxysteroid dehydrogenase (AKR1C14), and retinol dehydrogenase 2 (RDH2). Rat SRD5A1, AKR1C14, and RDH2 were cloned and expressed in COS1 cells, and the effects of butylated hydroxyanisole on these enzyme activities were measured. Butylated hydroxyanisole inhibited SRD5A1, AKR1C14, and RDH2 with IC50 values of 4.731±0.079μM, 5.753±0.073μM, and over 100μM, respectively. Butylated hydroxyanisole is a competitive inhibitor for both SRD5A1 and AKR1C14. Docking analysis shows that butylated hydroxyanisole binds to the dihydrotestosterone-binding site of AKR1C14. In conclusion, butylated hydroxyanisole is a potent inhibitor of SRD5A1 and AKR1C14, thus reducing the formation of active neurosteroids.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Rdh16Ratandrogen biosynthetic process  IDA  RGD 
Akr1c14Ratandrogen catabolic process  IDA  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Akr1c14Ratcytosol  IDA MMO:0000679RGD 

Objects Annotated

Genes (Rattus norvegicus)
Akr1c14  (aldo-keto reductase family 1, member C14)
Rdh16  (retinol dehydrogenase 16)


Additional Information