RGD Reference Report - Periodic mechanical stress enhances rat chondrocyte area expansion and migration through Src-PLCgamma1-ERK1/2 signaling. - Rat Genome Database

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Periodic mechanical stress enhances rat chondrocyte area expansion and migration through Src-PLCgamma1-ERK1/2 signaling.

Authors: Nong, Luming  Yin, Guoyong  Ren, Kewei  Tang, Jilei  Fan, Weimin 
Citation: Nong L, etal., Eur J Cell Biol. 2010 Sep;89(9):705-11. doi: 10.1016/j.ejcb.2010.04.005. Epub 2010 May 26.
RGD ID: 597621556
Pubmed: PMID:20537760   (View Abstract at PubMed)
DOI: DOI:10.1016/j.ejcb.2010.04.005   (Journal Full-text)

The signal transduction pathways of chondrocyte area expansion and migration under periodic mechanical stress remain a matter of debate. We explore this question by performing cell culture experiments in our self-developed periodic stress field and perfusion culture system. Under periodic mechanical stress, we find that both rat chondrocyte area and migration are significantly amplified. These changes are associated with increases in the phosphorylation of Src, PLCgamma1 and ERK1/2. Area expansion, migration and phosphorylation of PLCgamma1-Tyr(783) and ERK1/2-Thr(202)/Tyr(204) are inhibited (p<0.05) after pretreatment with Src inhibitor (PP2). We further demonstrate that area expansion, migration and phosphorylation of ERK1/2-Thr(202)/Tyr(204) are significantly inhibited (p<0.05) after pretreatment with PLCgamma1 inhibitor (U73122); the phosphorylation site of Src-Tyr(418) is not affected. After pretreatment with an ERK1/2 inhibitor (PD98059), area expansion and migration are inhibited (p<0.05), while the phosphorylation sites of Src-Tyr(418) and PLCgamma1-Tyr(783) are not affected. These findings suggest that periodic mechanical stress promotes chondrocyte area expansion and migration in part through the Src-PLCgamma1-ERK1/2 signaling pathway.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  

Objects Annotated

Genes (Rattus norvegicus)
Plcg1  (phospholipase C, gamma 1)
Src  (SRC proto-oncogene, non-receptor tyrosine kinase)


Additional Information