RGD Reference Report - Proteomics of microparticles after experimental pulmonary embolism. - Rat Genome Database

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Proteomics of microparticles after experimental pulmonary embolism.

Authors: Watts, JA  Lee, YY  Gellar, MA  Fulkerson, MB  Hwang, SI  Kline, JA 
Citation: Watts JA, etal., Thromb Res. 2011 Oct 17.
RGD ID: 5688769
Pubmed: PMID:22014850   (View Abstract at PubMed)
DOI: DOI:10.1016/j.thromres.2011.09.016   (Journal Full-text)

INTRODUCTION: Microparticles (MPs) are small fragments of apoptotic or activated cells that may contribute to pathological processes in cardiovascular diseases. In studies of MPs in clinical cohorts, it is unclear if observed changes in MP composition are a cause or a result of the cardiovascular disease being studied. The present studies employed a well-characterized rat model of experimental pulmonary embolism (PE) to determine if there were changes in MP characteristics as a result of pulmonary vascular occlusion. METHODS: PE was produced by infusing 25mum polystyrene microspheres into the jugular vein of anesthetized rats. MPs were isolated by differential centrifugation of arterial blood 18hr after PE. Proteins were separated by 1D gel electrophoresis and identified from tryptic digests by ultraperformance liquid chromatography (UPLC) coupled with tandem mass spectrometry. Statistical analysis was conducted using the Power Law Global Error Model (PLGEM). Changes in two proteins were confirmed by Western blot. RESULTS: Experimental PE produced pulmonary hypertension, mild systemic hypotension, hypoxia, hypercapnia and lactic acidosis. MPs showed significant elevation in proteins involved in clotting (fibronectin precursor, fibrinogen alpha, beta and gamma and von Willebrand factor) and several macroglobulin proteins, such as alpha-2-macroglobulin precursor compared with vehicle-treated control rats. Consistent with recent observations of hemolysis in PE, haptoglobin precursor protein, a major protein of hemoglobin clearance, decreased significantly in the PE animals. Plasma d-Dimer concentrations were significantly elevated, indicating that experimental PE produced a pro-coagulant state. CONCLUSIONS: These findings suggest that experimental PE produced significant, changes in MP characteristics to a prothrombotic phenotype.



Disease Annotations    Click to see Annotation Detail View
RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FGAHumanpulmonary embolism  ISOFga (Rattus norvegicus)protein:increased expression:blood microparticleRGD 
FGBHumanpulmonary embolism  ISOFgb (Rattus norvegicus)protein:increased expression:blood microparticleRGD 
FGGHumanpulmonary embolism  ISOFgg (Rattus norvegicus)protein:increased expression:blood microparticleRGD 
FgaRatpulmonary embolism  IEP protein:increased expression:blood microparticleRGD 
FgaMousepulmonary embolism  ISOFga (Rattus norvegicus)protein:increased expression:blood microparticleRGD 
FgbRatpulmonary embolism  IEP protein:increased expression:blood microparticleRGD 
FgbMousepulmonary embolism  ISOFgb (Rattus norvegicus)protein:increased expression:blood microparticleRGD 
FggRatpulmonary embolism  IEP protein:increased expression:blood microparticleRGD 
FggMousepulmonary embolism  ISOFgg (Rattus norvegicus)protein:increased expression:blood microparticleRGD 
HPHumanpulmonary embolism  ISOHp (Rattus norvegicus)protein:decreased expression:blood microparticleRGD 
HpRatpulmonary embolism  IEP protein:decreased expression:blood microparticleRGD 
HpMousepulmonary embolism  ISOHp (Rattus norvegicus)protein:decreased expression:blood microparticleRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FgaRatblood microparticle  IDA  RGD 
FgbRatblood microparticle  IDA  RGD 
FggRatblood microparticle  IDA  RGD 
HpRatblood microparticle  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Fga  (fibrinogen alpha chain)
Fgb  (fibrinogen beta chain)
Fgg  (fibrinogen gamma chain)
Hp  (haptoglobin)

Genes (Mus musculus)
Fga  (fibrinogen alpha chain)
Fgb  (fibrinogen beta chain)
Fgg  (fibrinogen gamma chain)
Hp  (haptoglobin)

Genes (Homo sapiens)
FGA  (fibrinogen alpha chain)
FGB  (fibrinogen beta chain)
FGG  (fibrinogen gamma chain)
HP  (haptoglobin)


Additional Information