RGD Reference Report - Silencing of Cited2 and Akap12 genes in radiation-induced rat osteosarcomas. - Rat Genome Database

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Silencing of Cited2 and Akap12 genes in radiation-induced rat osteosarcomas.

Authors: Daino, K  Roch-Lefevre, S  Ugolin, N  Altmeyer-Morel, S  Guilly, MN  Chevillard, S 
Citation: Daino K, etal., Biochem Biophys Res Commun. 2009 Dec 18;390(3):654-8. Epub 2009 Oct 13.
RGD ID: 5147850
Pubmed: PMID:19825367   (View Abstract at PubMed)
DOI: DOI:10.1016/j.bbrc.2009.10.022   (Journal Full-text)

We have previously studied genomic copy number changes and global gene expression patterns in rat osteosarcomas (OS) induced by the bone-seeking alpha emitter (238)Pu by comparative genomic hybridization (CGH) and oligonucleotide microarray analyses, respectively. Among the previously identified genes that were down-regulated in radiation-induced rat OS tumors, Cited2 (Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2) and Akap12 (a kinase anchoring protein, also known as src-suppressed C-kinase substrate, SSeCKS) genes mapped to the most frequently lost regions on chromosome 1p. In the present study, relative copy number losses of Cited2 and Akap12 genes were observed in 8 of 15 (53%) and 10 of 15 (67%) tumors by quantitative PCR analysis. Loss of Cited2 and Akap12 in the tumors was confirmed at the levels of mRNA and protein expression by quantitative RT-PCR and immunoblot analyses, respectively. These results indicate that Cited2 and Akap12 are silenced in radiation-induced OS, and therefore are novel candidate tumor-suppressor genes of this tumor.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AKAP12Humanosteosarcoma  ISORGD:70988mRNA,protein:decreased expression:osteoblast:RGD 
Akap12Ratosteosarcoma  IEP mRNA,protein:decreased expression:osteoblast:RGD 
Akap12Mouseosteosarcoma  ISORGD:70988mRNA,protein:decreased expression:osteoblast:RGD 
CITED2Humanosteosarcoma  ISORGD:620112DNA:deletion (rat)RGD 
Cited2Ratosteosarcoma  IAGP DNA:deletion (rat)RGD 
Cited2Mouseosteosarcoma  ISORGD:620112DNA:deletion (rat)RGD 

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Cited2Ratincreased osteosarcoma incidence  IAGP DNA:deletionRGD 
Objects Annotated

Genes (Rattus norvegicus)
Akap12  (A-kinase anchoring protein 12)
Cited2  (Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2)

Genes (Mus musculus)
Akap12  (A kinase anchor protein 12)
Cited2  (Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2)

Genes (Homo sapiens)
AKAP12  (A-kinase anchoring protein 12)
CITED2  (Cbp/p300 interacting transactivator with Glu/Asp rich carboxy-terminal domain 2)


Additional Information