RGD Reference Report - Rat liver phosphoribosyl pyrophosphate synthetase: existence of the purified enzyme as heterogeneous aggregates and identification of the catalytic subunit. - Rat Genome Database

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Rat liver phosphoribosyl pyrophosphate synthetase: existence of the purified enzyme as heterogeneous aggregates and identification of the catalytic subunit.

Authors: Kita, K  Otsuki, T  Ishizuka, T  Tatibana, M 
Citation: Kita K, etal., J Biochem. 1989 May;105(5):736-41.
RGD ID: 5134985
Pubmed: PMID:2546925   (View Abstract at PubMed)

Mammalian phosphoribosyl pyrophosphate (PRPP) synthetase has been extensively investigated. However, considerable ambiguity remains concerning its physical and regulatory properties. We purified PRPP synthetase from rat liver and studied some of the physical properties, in parallel with cloning experiments (Taira, M. et. al. [1987] J. Biol. Chem. 262, 14867-14870). 1) The enzyme was purified to a specific activity of 7,280 milliunits/mg, the highest value in the literature for a mammalian PRPP synthetase. The apparent molecular mass was over 1,000 kDa. 2) The final preparation contained 34-kDa, 38-kDa, and 40-kDa protein species, as analyzed by SDS gel electrophoresis. 3) Further attempts at separation using conventional procedures only led to a co-purification of the components. Thus, the purified enzyme appears to exist as complex aggregates composed of heterogeneous components. 4) Gel filtration of the enzyme in the presence of 1 M MgCl2 isolated part of the 34-kDa component, free of other species. The preparation was catalytically active, indicating that this component is the catalytic subunit. 5) The amino acid composition of the 34-kDa subunit and the amino acid sequences of its N-terminal region and of two tryptic peptides were determined. The results are in accord with the results of cDNA analyses.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Prps1RatAMP biosynthetic process  IDA  RGD 
Prps2RatAMP biosynthetic process  IDA  RGD 

Cellular Component

  

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Prps1RatATP binding  IDA  RGD 
Prps2RatATP binding  IDA  RGD 
Prps1Ratcarbohydrate binding  IDA  RGD 
Prps2Ratcarbohydrate binding  IDA  RGD 
Prps1Ratribose phosphate diphosphokinase activity  IDA  RGD 
Prps2Ratribose phosphate diphosphokinase activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Prps1  (phosphoribosyl pyrophosphate synthetase 1)
Prps2  (phosphoribosyl pyrophosphate synthetase 2)
Prpsap1  (phosphoribosyl pyrophosphate synthetase-associated protein 1)
Prpsap2  (phosphoribosyl pyrophosphate synthetase-associated protein 2)


Additional Information