RGD Reference Report - Development of relevant assay system to identify steroidogenic enzyme inhibitors. - Rat Genome Database

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Development of relevant assay system to identify steroidogenic enzyme inhibitors.

Authors: Nishizato, Y  Imai, S  Yabuki, M  Kido, H  Komuro, S 
Citation: Nishizato Y, etal., Toxicol In Vitro. 2010 Mar;24(2):677-85. Epub 2009 Aug 7.
RGD ID: 4891164
Pubmed: PMID:19665544   (View Abstract at PubMed)
DOI: DOI:10.1016/j.tiv.2009.07.037   (Journal Full-text)

Steroidogenesis in the adrenals, testes, and ovaries plays an important roles in hormonal homeostasis in vivo and is mediated by various enzymes, such as acid cholesterol esterase (CEase), neutral CEase, CYP11A, 3beta-hydroxysteroid dehydrogenase (HSD), CYP21, CYP11B, CYP17, CYP19 and 17beta-HSD. Compounds that are potent inhibitors of one or more steroidogenic enzymes can thus cause serious endocrine toxicity so that relevant assay systems for detecting such enzyme inhibition are useful for safety assessment. Methods already reported involve incubation of radiolabeled steroid precursors with enzyme sources followed by separation of products by TLC or other methods. However, it is unclear whether the systems in use are kinetically-optimized (i.e., reactions performed in the linear range in terms of both reaction time and substrate concentrations), so that they might not be applicable for appropriate inhibition studies. The purpose of this present study was to establish kinetically-optimized methods for rat adrenal, testis, and ovary tissues with radio-HPLC. After development of HPLC methods for separation of precursor substrates and derived metabolites, we investigated the time course and substrate concentration dependence of individual reactions, using radiolabeled substrates and enzyme sources (mitochondria-lysosomal, microsomal, and cytosolic fractions from rat adrenal, testis and ovary tissues). Linearity of metabolism was confirmed in terms of both reaction time and substrate concentrations, and we conclude that this approach can be utilized to assess the inhibition profiles of compounds impacting on steroidogenic enzyme activity.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Cyp11a1RatC21-steroid hormone biosynthetic process  IDA pregnenoloneRGD 
Cyp19a1Ratestrogen biosynthetic process  IDA estroneRGD 
Cyp11b1Ratglucocorticoid biosynthetic process  IDA corticosteroneRGD 
Cyp21a1Ratmineralocorticoid biosynthetic process  IDA 11-deoxycorticosteroneRGD 
Cyp21a1Ratprogesterone metabolic process  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cyp11a1  (cytochrome P450, family 11, subfamily a, polypeptide 1)
Cyp11b1  (cytochrome P450, family 11, subfamily b, polypeptide 1)
Cyp19a1  (cytochrome P450, family 19, subfamily a, polypeptide 1)
Cyp21a1  (cytochrome P450, family 21, subfamily a, polypeptide 1)


Additional Information