RGD Reference Report - Rat respiratory coronavirus infection: replication in airway and alveolar epithelial cells and the innate immune response. - Rat Genome Database

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Rat respiratory coronavirus infection: replication in airway and alveolar epithelial cells and the innate immune response.

Authors: Funk, CJ  Manzer, R  Miura, TA  Groshong, SD  Ito, Y  Travanty, EA  Leete, J  Holmes, KV  Mason, RJ 
Citation: Funk CJ, etal., J Gen Virol. 2009 Dec;90(Pt 12):2956-64. Epub 2009 Sep 9.
RGD ID: 4143520
Pubmed: PMID:19741068   (View Abstract at PubMed)
PMCID: PMC2887555   (View Article at PubMed Central)
DOI: DOI:10.1099/vir.0.014282-0   (Journal Full-text)

The rat coronavirus sialodacryoadenitis virus (SDAV) causes respiratory infection and provides a system for investigating respiratory coronaviruses in a natural host. A viral suspension in the form of a microspray aerosol was delivered by intratracheal instillation into the distal lung of 6-8-week-old Fischer 344 rats. SDAV inoculation produced a 7 % body weight loss over a 5 day period that was followed by recovery over the next 7 days. SDAV caused focal lesions in the lung, which were most severe on day 4 post-inoculation (p.i.). Immunofluorescent staining showed that four cell types supported SDAV virus replication in the lower respiratory tract, namely Clara cells, ciliated cells in the bronchial airway and alveolar type I and type II cells in the lung parenchyma. In bronchial alveolar lavage fluid (BALF) a neutrophil influx increased the population of neutrophils to 45 % compared with 6 % of the cells in control samples on day 2 after mock inoculation. Virus infection induced an increase in surfactant protein SP-D levels in BALF of infected rats on days 4 and 8 p.i. that subsided by day 12. The concentrations of chemokines MCP-1, LIX and CINC-1 in BALF increased on day 4 p.i., but returned to control levels by day 8. Intratracheal instillation of rats with SDAV coronavirus caused an acute, self-limited infection that is a useful model for studying the early events of the innate immune response to respiratory coronavirus infections in lungs of the natural virus host.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SFTPDHumanCoronavirus infectious disease  ISOSftpd (Rattus norvegicus)Severe Acute Respiratory Syndrome;protein:increased expression:lungRGD 
SftpdRatCoronavirus infectious disease  IEP Severe Acute Respiratory Syndrome;protein:increased expression:lungRGD 
SftpdMouseCoronavirus infectious disease  ISOSftpd (Rattus norvegicus)Severe Acute Respiratory Syndrome;protein:increased expression:lungRGD 
CXCL1Humansevere acute respiratory syndrome  ISOCxcl1 (Rattus norvegicus)protein:increased expression:respiratory system fluid/secretionRGD 
CXCL5Humansevere acute respiratory syndrome  ISOCxcl6 (Rattus norvegicus)protein:increased expression:respiratory fluid/secretionRGD 
Cxcl1Ratsevere acute respiratory syndrome  IEP protein:increased expression:respiratory system fluid/secretionRGD 
Cxcl1Mousesevere acute respiratory syndrome  ISOCxcl1 (Rattus norvegicus)protein:increased expression:respiratory system fluid/secretionRGD 
Cxcl5Mousesevere acute respiratory syndrome  ISOCxcl6 (Rattus norvegicus)protein:increased expression:respiratory fluid/secretionRGD 
Cxcl6Ratsevere acute respiratory syndrome  IEP protein:increased expression:respiratory fluid/secretionRGD 

Objects Annotated

Genes (Rattus norvegicus)
Cxcl1  (C-X-C motif chemokine ligand 1)
Cxcl6  (C-X-C motif chemokine ligand 6)
Sftpd  (surfactant protein D)

Genes (Mus musculus)
Cxcl1  (C-X-C motif chemokine ligand 1)
Cxcl5  (C-X-C motif chemokine ligand 5)
Sftpd  (surfactant associated protein D)

Genes (Homo sapiens)
CXCL1  (C-X-C motif chemokine ligand 1)
CXCL5  (C-X-C motif chemokine ligand 5)
SFTPD  (surfactant protein D)


Additional Information