RGD Reference Report - Characterization of Wistar-Kyoto rats showing hyperadiponectinemia. - Rat Genome Database

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Characterization of Wistar-Kyoto rats showing hyperadiponectinemia.

Authors: Inoue, T  Takemori, K  Yamamoto, K  Ito, H 
Citation: Inoue T, etal., Life Sci. 2010 Feb 27;86(9-10):344-50. Epub 2010 Jan 20.
RGD ID: 4142533
Pubmed: PMID:20093130   (View Abstract at PubMed)
DOI: DOI:10.1016/j.lfs.2010.01.003   (Journal Full-text)

AIMS: Wistar-Kyoto rats (HA-WKY) kept in the author's laboratory showed higher levels of serum adiponectin (approximately 4-fold) compared with Wistar-Kyoto/Izm rats (WKY/Izm), a WKY standard strain, at 6weeks old. In a preliminary study, HA-WKY but not WKY/Izm showed hyperinsulinemia and severe hypercholesterolemia when fed a high-fat diet. This study analyzed the differences between HA-WKY and WKY/Izm to investigate the causes of hyperadiponectinemia. MAIN METHODS: Six-week-old male HA-WKY and WKY/Izm were used for an analysis of adiponectin-related factors. KEY FINDINGS: The main intermediates in the adiponectin signaling pathway, AMP-activated protein kinase and peroxisome proliferator-activated receptor alpha, were activated at similar levels in liver and skeletal muscle between HA-WKY and WKY/Izm, although HA-WKY had not only higher adiponectin concentrations but also extremely high levels of high-molecular weight (HMW, polymer) adiponectin, which is the active form. The main difference between HA-WKY and WKY/Izm was the existence of adiponectin, mainly middle-molecular weight (MMW, hexamer) and HMW adiponectin multimers, in skeletal muscle extracts from WKY/Izm but not HA-WKY. Skeletal muscle in WKY/Izm expressed much higher amounts of T-cadherin, a receptor for MMW and HMW adiponectin multimers, than that in HA-WKY. In contrast, there was no significant difference in the expression level of adiponectin receptor 2 for trimer, MMW, and HMW adiponectin multimers. SIGNIFICANCE: The results suggested that the existence of adiponectin in WKY/Izm skeletal muscle was due to the binding of serum adiponectin. The difference in serum adiponectin concentrations between HA-WKY and WKY/Izm could come from the difference in adiponectin binding to skeletal muscle.

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Mammalian Phenotype
Objects Annotated

WKY/Kiha  (NA)

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