RGD Reference Report - Regulation of argininosuccinate synthetase mRNA level in rat foetal hepatocytes. - Rat Genome Database

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Regulation of argininosuccinate synthetase mRNA level in rat foetal hepatocytes.

Authors: Bourgeois, P  Harlin, JC  Renouf, S  Goutal, I  Fairand, A  Husson, A 
Citation: Bourgeois P, etal., Eur J Biochem. 1997 Nov 1;249(3):669-74.
RGD ID: 4139912
Pubmed: PMID:9395312   (View Abstract at PubMed)

Expression of the hepatic gene for argininosuccinate synthase (ASS), one of the key enzymes of the urea cycle, was analysed during the perinatal period in the rat. To this end, the amount of specific mRNA was measured in the liver at various stages of development and in cultured foetal hepatocytes maintained in different hormonal conditions. The ASS mRNA was first detected in 15.5-day foetuses and its level increased concomitantly with a rise in the enzyme activity, suggesting that the appearance of the ASS activity reflects the turning on of specific gene transcription. This was demonstrated by run-on assay which showed an enhanced rate of transcription of the ASS gene during the perinatal period. When foetal hepatocytes were cultured with dexamethasone, a dose-dependent increase in ASS mRNA was measured, which was completely abolished by actinomycin D addition. The transcription rate of the gene was increased about twofold in the presence of the steroid, as measured by nuclear run-on assay. This transcriptional action could additionally require a protein factor since it could be inhibited by the simultaneous addition of puromycin. Insulin or glucagon respectively repressed or enhanced the dexamethasone-induced accumulation of ASS mRNA when added simultaneously with the steroid for 24 h. This developmental regulation of the ASS mRNA by glucocorticoids, insulin and glucagon could account for the modulation of the enzyme activity previously observed in vivo and in vitro in the foetal liver.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ass1Ratcellular response to dexamethasone stimulus  IEP  RGD 
Ass1Ratcellular response to glucagon stimulus  IEP with dexamethasoneRGD 
Ass1Ratliver development  IEP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ass1  (argininosuccinate synthase 1)


Additional Information