RGD Reference Report - Relative CO(2)/NH(3) selectivities of mammalian aquaporins 0-9. - Rat Genome Database

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Relative CO(2)/NH(3) selectivities of mammalian aquaporins 0-9.

Authors: Geyer, R Ryan  Musa-Aziz, Raif  Qin, Xue  Boron, Walter F 
Citation: Geyer RR, etal., Am J Physiol Cell Physiol. 2013 May 15;304(10):C985-94. doi: 10.1152/ajpcell.00033.2013. Epub 2013 Mar 13.
RGD ID: 408345215
Pubmed: PMID:23485707   (View Abstract at PubMed)
DOI: DOI:10.1152/ajpcell.00033.2013   (Journal Full-text)

Previous work showed that aquaporin 1 (AQP1), AQP4-M23, and AQP5 each has a characteristic CO(2)/NH(3) and CO(2)/H(2)O permeability ratio. The goal of the present study is to characterize AQPs 0-9, which traffic to the plasma membrane when heterologously expressed in Xenopus oocytes. We use video microscopy to compute osmotic water permeability (P(f)) and microelectrodes to record transient changes in surface pH (ΔpH(S)) caused by CO(2) or NH(3) influx. Subtracting respective values for day-matched, H(2)O-injected control oocytes yields the channel-specific values P(f)* and ΔpH(S)*. We find that P(f)* is significantly >0 for all AQPs tested except AQP6. (ΔpH(S)*)(CO(2)) is significantly >0 for AQP0, AQP1, AQP4-M23, AQP5, AQP6, and AQP9. (ΔpH(S)*)(NH(3)) is >0 for AQP1, AQP3, AQP6, AQP7, AQP8, and AQP9. The ratio (ΔpH(S)*)(CO(2))/P(f)* falls in the sequence AQP6 (∞) > AQP5 > AQP4-M23 > AQP0 ≅ AQP1 ≅ AQP9 > others (0). The ratio (ΔpH(S)*)(NH(3))/P(f)* falls in the sequence AQP6 (∞) > AQP3 ≅ AQP7 ≅ AQP8 ≅ AQP9 > AQP1 > others (0). Finally, the ratio (ΔpH(S)*)(CO(2))/(-ΔpH(S)*)(NH(3)) falls in the sequence AQP0 (∞) ≅ AQP4-M23 ≅ AQP5 > AQP6 > AQP1 > AQP9 > AQP3 (0) ≅ AQP7 ≅ AQP8. The ratio (ΔpH(S)*)(CO(2))/(-ΔpH(S)*)(NH(3)) is indeterminate for both AQP2 and AQP4-M1. In summary, we find that mammalian AQPs exhibit a diverse range of selectivities for CO(2) vs. NH(3) vs. H(2)O. As a consequence, by expressing specific combinations of AQPs, cells could exert considerable control over the movements of each of these three substances.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Aqp6Ratammonium channel activity enablesIDA PMID:23485707UniProt 
Aqp6Ratcarbon dioxide transmembrane transporter activity enablesIDA PMID:23485707UniProt 
Aqp6Ratwater channel activity NOT|enablesIDA PMID:23485707UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Aqp6  (aquaporin 6)


Additional Information