RGD Reference Report - Cloning and functional expression of rAOP9L a new member of aquaporin family from rat liver. - Rat Genome Database

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Cloning and functional expression of rAOP9L a new member of aquaporin family from rat liver.

Authors: Ko, S B  Uchida, S  Naruse, S  Kuwahara, M  Ishibashi, K  Marumo, F  Hayakawa, T  Sasaki, S 
Citation: Ko SB, etal., Biochem Mol Biol Int. 1999 Feb;47(2):309-18. doi: 10.1080/15216549900201333.
RGD ID: 408345202
Pubmed: PMID:10205677   (View Abstract at PubMed)
DOI: DOI:10.1080/15216549900201333   (Journal Full-text)

A new aquaporin was isolated from rat liver based on homology to known aquaporins. A 1408 bp cDNA was sequenced (designated rAQP9L) with a 885 bp open reading frame encoding a 295 amino acid hydrophobic protein. rAQP9L has the greatest amino-acid sequence identity with human AQP9 (75%) and a less homology with AQP3 (49%) and AQP7 (47%). Northern blot analysis indicated a 1.4-kb transcript expressed strongly in liver > testis > brain = lung. Expression of rAQP9L cRNA in Xenopus oocytes increased osmotic water permeability by 6-folds which was inhibited by 0.3 mM mercury chloride by 42%. rAQP9L also facilitated glycerol and urea transport by 2- and 5-folds, respectively. The large discrepancy of tissue distribution between hAQP9 and rAQP9L suggest that rAQP9L is a new aquaporin, which is involved in transport of urea as well as water in liver.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Aqp9Ratglycerol channel activity enablesIDA PMID:10205677UniProt 
Aqp9Raturea channel activity enablesIDA PMID:10205677UniProt 
Aqp9Ratwater channel activity enablesIDA PMID:10205677UniProt 

Objects Annotated

Genes (Rattus norvegicus)
Aqp9  (aquaporin 9)


Additional Information