RGD Reference Report - Immunocytochemical Localization of Olfactory-signaling Molecules in Human and Rat Spermatozoa. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Behavioral & Substance Use Disorder Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources
Advanced Search (OLGA)

Immunocytochemical Localization of Olfactory-signaling Molecules in Human and Rat Spermatozoa.

Authors: Makeyeva, Yuliya  Nicol, Christopher  Ledger, William L  Ryugo, David K 
Citation: Makeyeva Y, etal., J Histochem Cytochem. 2020 Jul;68(7):491-513. doi: 10.1369/0022155420939833. Epub 2020 Jun 30.
RGD ID: 407550220
Pubmed: PMID:32603211   (View Abstract at PubMed)
PMCID: PMC7350079   (View Article at PubMed Central)
DOI: DOI:10.1369/0022155420939833   (Journal Full-text)

Expression of olfactory receptors (ORs) in non-olfactory tissues has been widely reported over the last 20 years. Olfactory marker protein (OMP) is highly expressed in mature olfactory sensory neurons (mOSNs) of the olfactory epithelium. It is involved in the olfactory signal transduction pathway, which is mediated by well-conserved components, including ORs, olfactory G protein (Golf), and adenylyl cyclase 3 (AC3). OMP is widely expressed in non-olfactory tissues with an apparent preference for motile cells. We hypothesized that OMP is expressed in compartment-specific locations and co-localize with an OR, Golf, and AC3 in rat epididymal and human-ejaculated spermatozoa. We used immunocytochemistry to examine the expression patterns of OMP and OR6B2 (human OR, served as positive olfactory control) in experimentally induced modes of activation and determine whether there are any observable differences in proteins expression during the post-ejaculatory stages of spermatozoal functional maturation. We found that OMP was expressed in compartment-specific locations in human and rat spermatozoa. OMP was co-expressed with Golf and AC3 in rat spermatozoa and with OR6B2 in all three modes of activation (control, activated, and hyperactivated), and the mode of activation changed the co-expression pattern in acrosomal-reacted human spermatozoa. These observations suggest that OMP expression is a reliable indicator of OR-mediated chemoreception, may be used to identify ectopically expressed ORs, and could participate in second messenger signaling cascades that mediate fertility.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Adcy3Ratacrosomal vesicle  IDA MMO:0000538RGD 
GnalRatacrosomal vesicle  IDA MMO:0000538RGD 
OmpRatacrosomal vesicle  IDA MMO:0000538RGD 
OmpRataxon  IDA MMO:0000538RGD 
OmpRatcytoplasm  IDA MMO:0000538RGD 
OmpRatdendrite  IDA MMO:0000538RGD 
OmpRatdendritic knob  IDA MMO:0000538RGD 
OmpRatneuronal cell body  IDA MMO:0000538RGD 
Adcy3Ratsperm midpiece  IDA MMO:0000538RGD 
GnalRatsperm midpiece  IDA MMO:0000538RGD 
OmpRatsperm midpiece  IDA MMO:0000538RGD 

Objects Annotated

Genes (Rattus norvegicus)
Adcy3  (adenylate cyclase 3)
Gnal  (G protein subunit alpha L)
Omp  (olfactory marker protein)


Additional Information