RGD Reference Report - Dynamics and nanoscale organization of the postsynaptic endocytic zone at excitatory synapses. - Rat Genome Database

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Dynamics and nanoscale organization of the postsynaptic endocytic zone at excitatory synapses.

Authors: Catsburg, Lisa Ae  Westra, Manon  van Schaik, Annemarie Ml  MacGillavry, Harold D 
Citation: Catsburg LA, etal., Elife. 2022 Jan 24;11:e74387. doi: 10.7554/eLife.74387.
RGD ID: 405650252
Pubmed: PMID:35072626   (View Abstract at PubMed)
PMCID: PMC8813055   (View Article at PubMed Central)
DOI: DOI:10.7554/eLife.74387   (Journal Full-text)

At postsynaptic sites of neurons, a prominent clathrin-coated structure, the endocytic zone (EZ), controls the trafficking of glutamate receptors and is essential for synaptic plasticity. Despite its importance, little is known about how this clathrin structure is organized to mediate endocytosis. We used live-cell and super-resolution microscopy to reveal the dynamic organization of this poorly understood clathrin structure in rat hippocampal neurons. We found that a subset of endocytic proteins only transiently appeared at postsynaptic sites. In contrast, other proteins were persistently enriched and partitioned at the edge of the EZ. We found that uncoupling the EZ from the synapse led to the loss of most of these components, while disrupting interactions with the actin cytoskeleton or membrane did not alter EZ positioning. Finally, we found that plasticity-inducing stimuli promoted the reorganization of the EZ. We conclude that the EZ is a stable, highly organized molecular platform where components are differentially recruited and positioned to orchestrate the endocytosis of synaptic receptors.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
AmphRatpostsynaptic endocytic zone is_active_inIDA PMID:35072626SynGO 
AmphRatpostsynaptic endocytic zone is_active_inIMP PMID:35072626SynGO 
Ap2b1Ratpostsynaptic endocytic zone is_active_inIDA PMID:35072626SynGO 
Ap2b1Ratpostsynaptic endocytic zone is_active_inIMP PMID:35072626SynGO 
CltaRatpostsynaptic endocytic zone is_active_inIDA PMID:35072626SynGO 
CltaRatpostsynaptic endocytic zone is_active_inIMP PMID:35072626SynGO 
Dnm2Ratpostsynaptic endocytic zone is_active_inIDA PMID:35072626SynGO 
Dnm2Ratpostsynaptic endocytic zone is_active_inIMP PMID:35072626SynGO 
Epn2Ratpostsynaptic endocytic zone is_active_inIDA PMID:35072626SynGO 
Epn2Ratpostsynaptic endocytic zone is_active_inIMP PMID:35072626SynGO 
Eps15Ratpostsynaptic endocytic zone is_active_inIDA PMID:35072626SynGO 
Eps15Ratpostsynaptic endocytic zone is_active_inIMP PMID:35072626SynGO 
Fcho1Ratpostsynaptic endocytic zone is_active_inIDA PMID:35072626SynGO 
Fcho1Ratpostsynaptic endocytic zone is_active_inIMP PMID:35072626SynGO 
Hip1rRatpostsynaptic endocytic zone is_active_inIDA PMID:35072626SynGO 
Hip1rRatpostsynaptic endocytic zone is_active_inIMP PMID:35072626SynGO 
Itsn1Ratpostsynaptic endocytic zone is_active_inIDA PMID:35072626SynGO 
Itsn1Ratpostsynaptic endocytic zone is_active_inIMP PMID:35072626SynGO 
PicalmRatpostsynaptic endocytic zone is_active_inIDA PMID:35072626SynGO 
PicalmRatpostsynaptic endocytic zone is_active_inIMP PMID:35072626SynGO 
Syne1Ratpostsynaptic endocytic zone is_active_inIDA PMID:35072626SynGO 
Syne1Ratpostsynaptic endocytic zone is_active_inIMP PMID:35072626SynGO 

Objects Annotated

Genes (Rattus norvegicus)
Amph  (amphiphysin)
Ap2b1  (adaptor related protein complex 2 subunit beta 1)
Clta  (clathrin, light chain A)
Dnm2  (dynamin 2)
Epn2  (epsin 2)
Eps15  (epidermal growth factor receptor pathway substrate 15)
Fcho1  (FCH and mu domain containing endocytic adaptor 1)
Hip1r  (huntingtin interacting protein 1 related)
Itsn1  (intersectin 1)
Picalm  (phosphatidylinositol binding clathrin assembly protein)
Syne1  (spectrin repeat containing nuclear envelope protein 1)


Additional Information