RGD Reference Report - Ethanol concentration-dependent alterations in gene expression during acute binge drinking in the HIV-1 transgenic rat. - Rat Genome Database

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Ethanol concentration-dependent alterations in gene expression during acute binge drinking in the HIV-1 transgenic rat.

Authors: Sarkar, Sraboni  Chang, Sulie L 
Citation: Sarkar S and Chang SL, Alcohol Clin Exp Res. 2013 Jul;37(7):1082-90. doi: 10.1111/acer.12077. Epub 2013 Feb 15.
RGD ID: 405100715
Pubmed: PMID:23413777   (View Abstract at PubMed)
PMCID: PMC3757312   (View Article at PubMed Central)
DOI: DOI:10.1111/acer.12077   (Journal Full-text)


BACKGROUND: Binge drinking of high ethanol (EtOH) concentration beverages is common among young adults and can be a risk factor for exposure to sexually transmitted diseases, including HIV-1. We used a novel noninfectious HIV-1 transgenic (HIV-1Tg) rat model that mimics HIV-1 patients in terms of altered immune responses and deficits in cognitive learning and memory to investigate EtOH concentration-dependent effects on 48 alcohol-modulated genes during binge EtOH administration.
METHODS: HIV-1Tg and control F344 rats were administered water, 8% EtOH, or 52% EtOH by gavage (i.g.) for 3 days (2.0 g/kg/d). Two hours after final treatment, blood, liver, and spleen were collected from each animal. Serum blood EtOH concentration (BEC) was measured, and gene expression in the liver and spleen was determined using a specifically designed PCR array.
RESULTS: The BEC was significantly higher in the 52% EtOH-treated HIV-1Tg rats compared with the 8% EtOH group; however, the BEC was higher in the 8% EtOH-treated control rats compared with the 52% EtOH group. There was no change in expression of the EtOH metabolism-related genes, Adh1, Adh4, and Cyp2e1, in either the 8 or 52% EtOH-treated HIV-1Tg rats, whereas expression of those genes was significantly higher in the liver of the 52% EtOH control rats, but not in the 8% EtOH group. In the HIV-1Tg rats, expression of the GABAA , metabotropic glutamate, and dopamine neurotransmitter receptor genes was significantly increased in the spleen of the 52% EtOH group, but not in the 8% EtOH group, whereas no change was observed in those genes in either of the control groups.
CONCLUSIONS: Our data indicate that, in the presence of HIV-1 infection, EtOH concentration-dependent binge drinking can have significantly different molecular effects.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
ADH1CHumanBinge Drinking  ISOAdh1c (Rattus norvegicus)mRNA:altered exxpression:liver (rat)RGD 
ADH4HumanBinge Drinking  ISOAdh4 (Rattus norvegicus)mRNA:altered exxpression:liver (rat)RGD 
Adh1MouseBinge Drinking  ISOAdh1c (Rattus norvegicus)mRNA:altered exxpression:liver (rat)RGD 
Adh1cRatBinge Drinking  IEP mRNA:altered exxpression:liver (rat)RGD 
Adh4RatBinge Drinking  IEP mRNA:altered exxpression:liver (rat)RGD 
Adh4MouseBinge Drinking  ISOAdh4 (Rattus norvegicus)mRNA:altered exxpression:liver (rat)RGD 
CYP2E1HumanBinge Drinking  ISOCyp2e1 (Rattus norvegicus)mRNA:altered exxpression:liver (rat)RGD 
Cyp2e1RatBinge Drinking  IEP mRNA:altered exxpression:liver (rat)RGD 
Cyp2e1MouseBinge Drinking  ISOCyp2e1 (Rattus norvegicus)mRNA:altered exxpression:liver (rat)RGD 
DRD1HumanBinge Drinking  ISODrd1 (Rattus norvegicus)associated with human immunodeficiency virus infectious disease more ...RGD 
DRD2HumanBinge Drinking  ISODrd2 (Rattus norvegicus)mRNA:altered exxpression:spleen (rat)RGD 
DRD3HumanBinge Drinking  ISODrd3 (Rattus norvegicus)associated with human immunodeficiency virus infectious disease more ...RGD 
DRD4HumanBinge Drinking  ISODrd4 (Rattus norvegicus)mRNA:altered exxpression:spleen (rat)RGD 
Drd1RatBinge Drinking  IEP associated with human immunodeficiency virus infectious disease more ...RGD 
Drd1MouseBinge Drinking  ISODrd1 (Rattus norvegicus)associated with human immunodeficiency virus infectious disease more ...RGD 
Drd2RatBinge Drinking  IEP mRNA:altered exxpression:spleen (rat)RGD 
Drd2MouseBinge Drinking  ISODrd2 (Rattus norvegicus)mRNA:altered exxpression:spleen (rat)RGD 
Drd3RatBinge Drinking  IEP associated with human immunodeficiency virus infectious disease more ...RGD 
Drd3MouseBinge Drinking  ISODrd3 (Rattus norvegicus)associated with human immunodeficiency virus infectious disease more ...RGD 
Drd4RatBinge Drinking  IEP mRNA:altered exxpression:spleen (rat)RGD 
Drd4MouseBinge Drinking  ISODrd4 (Rattus norvegicus)mRNA:altered exxpression:spleen (rat)RGD 
F344-Tg(HIV)1HsdRatBinge Drinking  IMP compared to wild type F344 controlsRGD 
GABRA1HumanBinge Drinking  ISOGabra1 (Rattus norvegicus)associated with human immunodeficiency virus infectious disease more ...RGD 
GABRA2HumanBinge Drinking  ISOGabra2 (Rattus norvegicus)associated with human immunodeficiency virus infectious disease more ...RGD 
GABRA3HumanBinge Drinking  ISOGabra3 (Rattus norvegicus)associated with human immunodeficiency virus infectious disease more ...RGD 
GABRA6HumanBinge Drinking  ISOGabra6 (Rattus norvegicus)mRNA:altered exxpression:spleen (rat)RGD 
GRM2HumanBinge Drinking  ISOGrm2 (Rattus norvegicus)associated with human immunodeficiency virus infectious disease more ...RGD 
GRM5HumanBinge Drinking  ISOGrm5 (Rattus norvegicus)associated with human immunodeficiency virus infectious disease more ...RGD 
Gabra1RatBinge Drinking  IEP associated with human immunodeficiency virus infectious disease more ...RGD 
Gabra1MouseBinge Drinking  ISOGabra1 (Rattus norvegicus)associated with human immunodeficiency virus infectious disease more ...RGD 
Gabra2RatBinge Drinking  IEP associated with human immunodeficiency virus infectious disease more ...RGD 
Gabra2MouseBinge Drinking  ISOGabra2 (Rattus norvegicus)associated with human immunodeficiency virus infectious disease more ...RGD 
Gabra3RatBinge Drinking  IEP associated with human immunodeficiency virus infectious disease more ...RGD 
Gabra3MouseBinge Drinking  ISOGabra3 (Rattus norvegicus)associated with human immunodeficiency virus infectious disease more ...RGD 
Gabra6RatBinge Drinking  IEP mRNA:altered exxpression:spleen (rat)RGD 
Gabra6MouseBinge Drinking  ISOGabra6 (Rattus norvegicus)mRNA:altered exxpression:spleen (rat)RGD 
Grm2RatBinge Drinking  IEP associated with human immunodeficiency virus infectious disease more ...RGD 
Grm2MouseBinge Drinking  ISOGrm2 (Rattus norvegicus)associated with human immunodeficiency virus infectious disease more ...RGD 
Grm5RatBinge Drinking  IEP associated with human immunodeficiency virus infectious disease more ...RGD 
Grm5MouseBinge Drinking  ISOGrm5 (Rattus norvegicus)associated with human immunodeficiency virus infectious disease more ...RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Adh1cRatresponse to ethanol  IEP  RGD 
Adh4Ratresponse to ethanol  IEP  RGD 
Cyp2e1Ratresponse to ethanol  IEP  RGD 
Drd2Ratresponse to ethanol  IEP  RGD 
Drd4Ratresponse to ethanol  IEP  RGD 
Gabra6Ratresponse to ethanol  IEP  RGD 

Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
F344-Tg(HIV)1HsdRatabnormal ethanol metabolism  IMP compared to wild type F344 controlsRGD 
Objects Annotated

Genes (Rattus norvegicus)
Adh1c  (alcohol dehydrogenase 1C (class I), gamma polypeptide)
Adh4  (alcohol dehydrogenase 4 (class II), pi polypeptide)
Cyp2e1  (cytochrome P450, family 2, subfamily e, polypeptide 1)
Drd1  (dopamine receptor D1)
Drd2  (dopamine receptor D2)
Drd3  (dopamine receptor D3)
Drd4  (dopamine receptor D4)
Gabra1  (gamma-aminobutyric acid type A receptor subunit alpha 1)
Gabra2  (gamma-aminobutyric acid type A receptor subunit alpha 2)
Gabra3  (gamma-aminobutyric acid type A receptor subunit alpha 3)
Gabra6  (gamma-aminobutyric acid type A receptor subunit alpha6)
Grm2  (glutamate metabotropic receptor 2)
Grm5  (glutamate metabotropic receptor 5)

Genes (Mus musculus)
Adh1  (alcohol dehydrogenase 1 (class I))
Adh4  (alcohol dehydrogenase 4 (class II), pi polypeptide)
Cyp2e1  (cytochrome P450, family 2, subfamily e, polypeptide 1)
Drd1  (dopamine receptor D1)
Drd2  (dopamine receptor D2)
Drd3  (dopamine receptor D3)
Drd4  (dopamine receptor D4)
Gabra1  (gamma-aminobutyric acid type A receptor subunit alpha 1)
Gabra2  (gamma-aminobutyric acid type A receptor subunit alpha 2)
Gabra3  (gamma-aminobutyric acid type A receptor subunit alpha 3)
Gabra6  (gamma-aminobutyric acid type A receptor subunit alpha 6)
Grm2  (glutamate receptor, metabotropic 2)
Grm5  (glutamate receptor, metabotropic 5)

Genes (Homo sapiens)
ADH1C  (alcohol dehydrogenase 1C (class I), gamma polypeptide)
ADH4  (alcohol dehydrogenase 4 (class II), pi polypeptide)
CYP2E1  (cytochrome P450 family 2 subfamily E member 1)
DRD1  (dopamine receptor D1)
DRD2  (dopamine receptor D2)
DRD3  (dopamine receptor D3)
DRD4  (dopamine receptor D4)
GABRA1  (gamma-aminobutyric acid type A receptor subunit alpha1)
GABRA2  (gamma-aminobutyric acid type A receptor subunit alpha2)
GABRA3  (gamma-aminobutyric acid type A receptor subunit alpha3)
GABRA6  (gamma-aminobutyric acid type A receptor subunit alpha6)
GRM2  (glutamate metabotropic receptor 2)
GRM5  (glutamate metabotropic receptor 5)

Strains
F344-Tg(HIV)1Hsd  (NA)


Additional Information