RGD Reference Report - Nerve growth factor continuously elevates in a rat rotator cuff tear model. - Rat Genome Database

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Nerve growth factor continuously elevates in a rat rotator cuff tear model.

Authors: Nagura, Naoshige  Kenmoku, Tomonori  Uchida, Kentaro  Nakawaki, Mitsufumi  Inoue, Gen  Takaso, Masashi 
Citation: Nagura N, etal., J Shoulder Elbow Surg. 2019 Jan;28(1):143-148. doi: 10.1016/j.jse.2018.06.030. Epub 2018 Aug 23.
RGD ID: 402463951
Pubmed: PMID:30146165   (View Abstract at PubMed)
DOI: DOI:10.1016/j.jse.2018.06.030   (Journal Full-text)


BACKGROUND: Nerve growth factor (NGF) plays a key role in osteoarthritic pain and low back pain. Rotator cuff tear (RCT) is often associated with severe shoulder pain. However, the role of NGF in RCT remains to be fully understood.
METHODS: Rats were divided into sham and RCT groups. The rotator cuff was harvested from the sham and RCT groups on various days for reverse transcription-polymerase chain reaction analysis of Tnfa, Ngf, Il1b, and Cox2 expression. Rotator cuffs from the sham and RCT groups were also harvested at 1 and 14 days for enzyme-linked immunosorbent assay and immunohistochemistry to assess NGF protein levels and localization. Rotator cuff-derived cells were stimulated with rat recombinant tumor necrosis factor (TNF)-α to investigate the involvement of TNF-α in the regulation of NGF expression.
RESULTS: Tnfa and Ngf messenger RNA levels increased within 1 day in the RCT group. Notably, Tnfa and Ngf upregulation persisted for up to 56 days after the RCT surgery, while Il1b and Cox2 expression was significantly reduced. NGF levels in the RCT group were significantly higher than those in the sham operation group on days 1 and 14. Certain inflammatory cells and synovial-like cells lining the surface of the laminated tears were NGF-positive on days 1 and 14, respectively. Ngf messenger RNA levels increased significantly in rotator cuff-derived cells after TNF-α stimulation.
CONCLUSION: NGF levels are continuously elevated in RCT, which is mainly regulated by TNF-α. NGF may thus represent a potential target for therapies that modulate RCT pain.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
NGFHumanRotator Cuff Injuries  ISONgf (Rattus norvegicus)mRNA and protein:increased expression:rotator cuffRGD 
NgfMouseRotator Cuff Injuries  ISONgf (Rattus norvegicus)mRNA and protein:increased expression:rotator cuffRGD 
NgfRatRotator Cuff Injuries  IEP mRNA and protein:increased expression:rotator cuffRGD 

Objects Annotated

Genes (Rattus norvegicus)
Ngf  (nerve growth factor)

Genes (Mus musculus)
Ngf  (nerve growth factor)

Genes (Homo sapiens)
NGF  (nerve growth factor)


Additional Information