RGD Reference Report - ESR2 regulates granulosa cell genes essential for follicle maturation and ovulation. - Rat Genome Database

Send us a Message
Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   
Search RGD Grant Resources Citing RGD About Us Contact Us
Genes Variants Community Projects QTLs Strains Markers Genome Information Ontologies Cell Lines References Download Submit Data
OntoMate (Literature Search) JBrowse (Genome Browser) Synteny Browser (VCMap) Variant Visualizer Multi-Ontology Enrichment (MOET) Gene-Ortholog Location Finder (GOLF) InterViewer (Protein-Protein Interactions) PhenoMiner (Quatitative Phenotypes) Gene Annotator OLGA (Gene List Generator) AllianceMine GViewer (Genome Viewer)
Aging & Age-Related Disease Cancer & Neoplastic Disease Cardiovascular Disease Coronavirus Disease Developmental Disease Diabetes Hematologic Disease Immune & Inflammatory Disease Infectious Disease Liver Disease Neurological Disease Obesity & Metabolic Syndrome Renal Disease Respiratory Disease Sensory Organ Disease
Find Models Genetic Models Autism Models Rat PhenoMiner (Quantitative Phenotypes) Chinchilla PhenoMiner Expected Ranges (Quantitative Phenotype) PhenoMiner Term Comparison Hybrid Rat Diversity Panel Phenotypes Phenotypes in Other Animal Models Animal Husbandry Strain Medical Records Phylogenetics Strain Availability Calendar Rats 101 Submissions Photo Archive
Pathways
Rat Community Forum Directory of Rat Laboratories Video Tutorials News RGD Publications RGD Presentations Archive Nomenclature Guidelines Resource Links Laboratory Resources Employment Resources
Advanced Search (OLGA)

ESR2 regulates granulosa cell genes essential for follicle maturation and ovulation.

Authors: Khristi, Vincentaben  Chakravarthi, V Praveen  Singh, Prabhakar  Ghosh, Subhra  Pramanik, Archit  Ratri, Anamika  Borosha, Shaon  Roby, Katherine F  Wolfe, Michael W  Rumi, M A Karim 
Citation: Khristi V, etal., Mol Cell Endocrinol. 2018 Oct 15;474:214-226. doi: 10.1016/j.mce.2018.03.012. Epub 2018 Mar 24.
RGD ID: 38548925
Pubmed: PMID:29580824   (View Abstract at PubMed)
DOI: DOI:10.1016/j.mce.2018.03.012   (Journal Full-text)

Estrogen receptor 2 (ESR2) plays a critical role in folliculogenesis and ovulation. Disruption of ESR2-function in the rats results in female infertility due to failure of ovulation. Ovulation failure occurred in two distinct rat models, a null mutant and a DNA binding domain (DBD) mutant of ESR2, indicating that transcriptional regulation by ESR2 is indispensable for ovulation. To define the regulatory role of ESR2 in preovulatory follicular maturation and ovulation, we investigated ovarian responsiveness to exogenous gonadotropins in prepubertal females. Granulosa cells (GCs) play a vital role in follicle maturation and ovulation, and ESR2-dependent estrogen signaling is predominant in GCs, therefore, we examined the differential expression of gonadotropin-induced genes in GCs. Of 32,623 genes detected by RNA-sequencing, 1696 were differentially expressed in Esr2-mutant rats (789 downregulated, and 907 upregulated, absolute fold change 2, FDR p < 0.05). Molecular pathway analyses indicated that these differentially expressed genes are involved in steroidogenesis, follicle maturation, and ovulation. Many of these genes are known regulators of ovarian function and a subset were also disrupted in Esr2-mutant mice. Interestingly, Kiss1 was identified as one of the differentially expressed genes implicating a potential role within the follicle and its regulation by ESR2. Our findings indicate that ESR2 regulates key genes in GCs that are essential for follicle maturation and ovulation in the rat.



Phenotype Annotations    Click to see Annotation Detail View

Mammalian Phenotype

Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Esr2Ratabnormal circulating androgen level  IMP  RGD 
Esr2em1SoarRatabnormal circulating androgen level  IMP compared to wild typeRGD 
SD-Esr2em1SoarRatabnormal circulating androgen level inducedIMPpeptide hormonecompared to wild typeRGD 
Esr2Ratabsent corpus luteum  IMP  RGD 
Esr2em1SoarRatabsent corpus luteum  IMP  RGD 
SD-Esr2em1SoarRatabsent corpus luteum  IMP  RGD 
SD-Esr2em2SoarRatabsent corpus luteum  IMP  RGD 
Esr2Ratanovulation  IMP  RGD 
Esr2em1SoarRatanovulation  IMP compared to wild typeRGD 
SD-Esr2em1SoarRatanovulation inducedIMPpeptide hormonecompared to wild typeRGD 
SD-Esr2em1SoarRatanovulation  IMP  RGD 
SD-Esr2em2SoarRatanovulation inducedIMPpeptide hormonecompared to wild-typeRGD 
SD-Esr2em2SoarRatanovulation  IMP  RGD 
Esr2Ratdecreased circulating progesterone level  IMP  RGD 
Esr2em1SoarRatdecreased circulating progesterone level  IMP compared to wild typeRGD 
SD-Esr2em1SoarRatdecreased circulating progesterone level inducedIMPpeptide hormonecompared to wild typeRGD 
SD-Esr2em2SoarRatdecreased circulating progesterone level inducedIMPpeptide hormonecompared to wild-typeRGD 
Esr2Ratdecreased ovary weight  IMP  RGD 
Esr2em1SoarRatdecreased ovary weight  IMP compared to wild typeRGD 
SD-Esr2em1SoarRatdecreased ovary weight inducedIMPpeptide hormonecompared to wild typeRGD 
SD-Esr2em2SoarRatdecreased ovary weight inducedIMPpeptide hormonecompared to wild-typeRGD 
Esr2Ratfemale infertility  IMP  RGD 
Esr2em1SoarRatfemale infertility  IMP  RGD 
SD-Esr2em1SoarRatfemale infertility  IMP  RGD 
SD-Esr2em2SoarRatfemale infertility  IMP  RGD 
Objects Annotated

Genes (Rattus norvegicus)
Esr2  (estrogen receptor 2)
Esr2em1Soar  (estrogen receptor 2; ZFN induced mutant 1, Soar)

Strains
SD-Esr2em1Soar  (NA)
SD-Esr2em2Soar  (NA)


Additional Information