RGD Reference Report - Expression of IRAK‑3 is associated with colitis‑associated tumorigenesis in mice. - Rat Genome Database

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Expression of IRAK‑3 is associated with colitis‑associated tumorigenesis in mice.

Authors: Xu, Dingting  Zhang, Hanyun  Wang, Xiaoying  Chen, Yan 
Citation: Xu D, etal., Mol Med Rep. 2017 Sep;16(3):3415-3420. doi: 10.3892/mmr.2017.6958. Epub 2017 Jul 12.
RGD ID: 34888231
Pubmed: PMID:28713897   (View Abstract at PubMed)
DOI: DOI:10.3892/mmr.2017.6958   (Journal Full-text)

Interleukin‑1 receptor‑associated kinase‑3 (IRAK‑3) is a negative regulator in Toll‑like receptor (TLR) pathways. The present study investigated the importance of IRAK‑3 in a mouse model of chemically‑induced colitis‑associated tumorigenesis. The colitis‑associated tumorigenesis was induced in ICR mice by the administration of 1,2‑dimethyl hydrazine (DMH) and dextran sodium sulfate (DSS), termed the DMH + DSS group. In the DSS group, mice were administered with DSS; in the DMH group, mice were injected with DMH; in the control group, mice were injected with physiological saline. The clinical signs were examined for 20 weeks; tissue samples were analyzed at week 4, 9, 13 and 20. At week 20, the levels of IRAK‑3 were analyzed using immunohistochemistry, western blot analysis, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) analysis, and methylation‑specific PCR. At week 20, the DMH + DSS group mice exhibited a decrease in total body weight and had developed canalicular adenoma or adenocarcinoma. The mice in the DSS group and DMH group presented with significant colitis at week 20. The mice with colitis‑associated tumorigenesis were found to have decreased levels of IRAK‑3, compared with the mice in the other groups, as evidenced by the results of the immunohistochemistry (P=0.002), RT‑qPCR analysis (P<0.001) and western blot analysis (P<0.001). IRAK‑3 methylation was observed in all experimental groups. Taken together, DMH + DSS induction in colitis led to increased inflammation and risk of tumorigenesis. IRAK‑3 methylation may be a predictive factor in the transition from colitis to cancer.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
IRAK3HumanGlandular and Epithelial Neoplasms  ISOIrak3 (Mus musculus)associated with ulcerative colitis more ...RGD 
Irak3RatGlandular and Epithelial Neoplasms  ISOIrak3 (Mus musculus)associated with ulcerative colitis more ...RGD 
Irak3MouseGlandular and Epithelial Neoplasms  IEP associated with ulcerative colitis more ...RGD 

Gene-Chemical Interaction Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
IRAK3Human1,2-dimethylhydrazine decreases expression ISOIrak3 (Mus musculus)1 and 2-dimethylhydrazine decreases expression of Irak3 mRNA and protein in colonRGD 
IRAK3Human1,2-dimethylhydrazine decreases expressionISOIrak3 (Mus musculus)1 and 2-dimethylhydrazine decreases expression of Irak3 mRNA and protein in colonRGD 
Irak3Rat1,2-dimethylhydrazine decreases expressionISOIrak3 (Mus musculus)1 and 2-dimethylhydrazine decreases expression of Irak3 mRNA and protein in colonRGD 
Irak3Rat1,2-dimethylhydrazine decreases expression ISOIrak3 (Mus musculus)1 and 2-dimethylhydrazine decreases expression of Irak3 mRNA and protein in colonRGD 
Irak3Mouse1,2-dimethylhydrazine decreases expressionEXP 1 and 2-dimethylhydrazine decreases expression of Irak3 mRNA and protein in colonRGD 
Irak3Mouse1,2-dimethylhydrazine decreases expression EXP 1 and 2-dimethylhydrazine decreases expression of Irak3 mRNA and protein in colonRGD 
IRAK3Humansodium sulfate decreases expression ISOIrak3 (Mus musculus)sodium sulfate decreases expression of Irak3 mRNA and protein in colonRGD 
Irak3Ratsodium sulfate decreases expression ISOIrak3 (Mus musculus)sodium sulfate decreases expression of Irak3 mRNA and protein in colonRGD 
Irak3Mousesodium sulfate decreases expression EXP sodium sulfate decreases expression of Irak3 mRNA and protein in colonRGD 

Objects Annotated

Genes (Rattus norvegicus)
Irak3  (interleukin-1 receptor-associated kinase 3)

Genes (Mus musculus)
Irak3  (interleukin-1 receptor-associated kinase 3)

Genes (Homo sapiens)
IRAK3  (interleukin 1 receptor associated kinase 3)


Additional Information