RGD Reference Report - Preservation of myocardial fatty acid oxidation prevents diastolic dysfunction in mice subjected to angiotensin II infusion. - Rat Genome Database

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Preservation of myocardial fatty acid oxidation prevents diastolic dysfunction in mice subjected to angiotensin II infusion.

Authors: Choi, Yong Seon  de Mattos, Ana Barbosa Marcondes  Shao, Dan  Li, Tao  Nabben, Miranda  Kim, Maengjo  Wang, Wang  Tian, Rong  Kolwicz, Stephen C 
Citation: Choi YS, etal., J Mol Cell Cardiol. 2016 Nov;100:64-71. doi: 10.1016/j.yjmcc.2016.09.001. Epub 2016 Sep 28.
RGD ID: 329955570
Pubmed: PMID:27693463   (View Abstract at PubMed)
PMCID: PMC5154855   (View Article at PubMed Central)
DOI: DOI:10.1016/j.yjmcc.2016.09.001   (Journal Full-text)


RATIONALE: Diastolic dysfunction is a common feature in many heart failure patients with preserved ejection fraction and has been associated with altered myocardial metabolism in hypertensive and diabetic patients. Therefore, metabolic interventions to improve diastolic function are warranted. In mice with a germline cardiac-specific deletion of acetyl CoA carboxylase 2 (ACC2), systolic dysfunction induced by pressure-overload was prevented by maintaining cardiac fatty acid oxidation (FAO). However, it has not been evaluated whether this strategy would prevent the development of diastolic dysfunction in the adult heart.
OBJECTIVE: To test the hypothesis that augmenting cardiac FAO is protective against angiotensin II (AngII)-induced diastolic dysfunction in an adult mouse heart.
METHODS AND RESULTS: We generated a mouse model to induce cardiac-specific deletion of ACC2 in adult mice. Tamoxifen treatment (20mg/kg/day for 5days) was sufficient to delete ACC2 protein and increase cardiac FAO by 50% in ACC2 flox/flox-MerCreMer+ mice (iKO). After 4weeks of AngII (1.1mg/kg/day), delivered by osmotic mini-pumps, iKO mice showed normalized E/E' and E'/A' ratios compared to AngII treated controls (CON). The prevention of diastolic dysfunction in iKO-AngII was accompanied by maintained FAO and reduced glycolysis and anaplerosis. Furthermore, iKO-AngII hearts had a~50% attenuation of cardiac hypertrophy and fibrosis compared to CON. In addition, maintenance of FAO in iKO hearts suppressed AngII-associated increases in oxidative stress and sustained mitochondrial respiratory complex activities.
CONCLUSION: These data demonstrate that impaired FAO is a contributor to the development of diastolic dysfunction induced by AngII. Maintenance of FAO in this model leads to an attenuation of hypertrophy, reduces fibrosis, suppresses increases in oxidative stress, and maintains mitochondrial function. Therefore, targeting mitochondrial FAO is a promising therapeutic strategy for the treatment of diastolic dysfunction.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Diastolic Dysfunction  ISOAcacb (Mus musculus)329955570; 329955570 RGD 
Diastolic Dysfunction  IMP 329955570 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Acacb  (acetyl-CoA carboxylase beta)

Genes (Mus musculus)
Acacb  (acetyl-Coenzyme A carboxylase beta)

Genes (Homo sapiens)
ACACB  (acetyl-CoA carboxylase beta)


Additional Information