RGD Reference Report - Glutamate stimulates oligodendrocyte progenitor migration mediated via an alphav integrin/myelin proteolipid protein complex. - Rat Genome Database

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Glutamate stimulates oligodendrocyte progenitor migration mediated via an alphav integrin/myelin proteolipid protein complex.

Authors: Gudz, Tatyana I  Komuro, Hitoshi  Macklin, Wendy B 
Citation: Gudz TI, etal., J Neurosci. 2006 Mar 1;26(9):2458-66. doi: 10.1523/JNEUROSCI.4054-05.2006.
RGD ID: 30296668
Pubmed: PMID:16510724   (View Abstract at PubMed)
PMCID: PMC6793653   (View Article at PubMed Central)
DOI: DOI:10.1523/JNEUROSCI.4054-05.2006   (Journal Full-text)

In the mammalian CNS, oligodendrocyte precursor cells (OPCs) express most neurotransmitter receptors, but their function remains unclear. The current studies suggest a physiological role for glutamate (AMPA and/or kainate) receptors in OPC migration. AMPA stimulated alphav integrin-mediated OPC migration by increasing both the rate of cell movement and the frequency of Ca2+ transients. A protein complex containing the myelin proteolipid protein (PLP) and alphav integrin modulated the AMPA-stimulated migration, and stimulation of OPC AMPA receptors resulted in increased association of the AMPA receptor subunits themselves with the alphav integrin/PLP complex. Thus, after AMPA receptor stimulation, an alphav integrin/PLP/neurotransmitter receptor protein complex forms that reduces binding to the extracellular matrix and enhances OPC migration. To assess the extent to which PLP was involved in the AMPA-stimulated migration, OPCs from the myelin-deficient (MD) rat, which has a PLP gene mutation, were analyzed. OPCs from the MD rat had a normal basal migration rate, but AMPA did not stimulate the migration of these cells, suggesting that the PLP/alphav integrin complex was important for the AMPA-mediated induction. AMPA-induced modulation of OPC migration was abolished by pertussis toxin, although baseline migration was normal. Thus, G-protein-dependent signaling is crucial for AMPA-stimulated migration of OPCs but not for basal OPC migration. Other signaling pathways involved in this AMPA-stimulated OPC migration were also determined. These studies highlight novel signaling determinants of OPC migration and suggest that glutamate could play a pivotal role in regulating integrin-mediated OPC migration.



Gene-Chemical Interaction Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PLP1Human2,3-Dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline decreases bindingISOPlp1 (Rattus norvegicus)NBQX decreases binding of Plp1 to Itgav protein in oligodendrocyte progenitor cellsRGD 
Plp1Rat2,3-Dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline decreases bindingEXP NBQX decreases binding of Plp1 to Itgav protein in oligodendrocyte progenitor cellsRGD 
Plp1Mouse2,3-Dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline decreases bindingISOPlp1 (Rattus norvegicus)NBQX decreases binding of Plp1 to Itgav protein in oligodendrocyte progenitor cellsRGD 
PLP1HumanAMPA receptor agonist increases bindingISOPlp1 (Rattus norvegicus)AMPA receptor agonist increases binding of Plp1 to Gria2 more ...RGD 
Plp1RatAMPA receptor agonist increases bindingEXP AMPA receptor agonist increases binding of Plp1 to Gria2 more ...RGD 
Plp1MouseAMPA receptor agonist increases bindingISOPlp1 (Rattus norvegicus)AMPA receptor agonist increases binding of Plp1 to Gria2 more ...RGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Plp1RatAMPA selective glutamate receptor signaling pathway  IMP  RGD 
Plp1Ratpositive regulation of calcium ion transmembrane transport  IMP  RGD 
Plp1Ratpositive regulation of cell migration  IMP  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Plp1Ratintegrin alphav-beta3 complex  IDA co-immunoprecipitation MMO:0000683RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Plp1Ratprotein-containing complex binding  IPIGria2 (Rattus norvegicus) more ... RGD 

Objects Annotated

Genes (Rattus norvegicus)
Plp1  (proteolipid protein 1)

Genes (Mus musculus)
Plp1  (proteolipid protein (myelin) 1)

Genes (Homo sapiens)
PLP1  (proteolipid protein 1)


Additional Information