RGD Reference Report - MicroRNA-155 regulates the Th17 immune response by targeting Ets-1 in Behçet's disease. - Rat Genome Database

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MicroRNA-155 regulates the Th17 immune response by targeting Ets-1 in Behçet's disease.

Authors: Na, So Young  Park, Mi-Jin  Park, Sun  Lee, Eun-So 
Citation: Na SY, etal., Clin Exp Rheumatol. 2016 Sep-Oct;34(6 Suppl 102):S56-S63. Epub 2016 Apr 18.
RGD ID: 25671481
Pubmed: PMID:27156371   (View Abstract at PubMed)

OBJECTIVES: The goal of this study was to investigate whether microRNA-155 might be a potential therapeutic target for Behçet's disease (BD).
METHODS: Expression levels of miR-155 were quantified using TaqMan microRNA assays in peripheral blood mononuclear cells and in isolated CD4+ T cells from BD patients and healthy controls. To identify targets of miR-155, luciferase reporter assays and western blotting were performed. The effect of miR-155 on Th17 polarisation was analysed in patients with active BD by using flow cytometry and enzyme-linked immunosorbent assay.
RESULTS: The expression of miR-155 and IL-17 was significantly increased in CD4+ T cells of patients with active BD. A luciferase reporter assay and western blot showed that Ets-1 expression was reduced by miR-155 mimics. Furthermore, the expression of Ets-1 was significantly decreased in patients with active BD compared to healthy controls. More importantly, repression of miR-155 in CD4+ T cells from active BD patients increased Ets-1 expression and reduced the number of IL- 17-expressing T cells and overall IL-17 production.
CONCLUSIONS: MiR-155 regulates the Th17 immune response by targeting Ets-1. Suppression of miR-155 reduced the amount of pathogenic IL-17-expressing T cells and may be a potential therapeutic strategy for BD.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Behcet's disease  IEP 25671481 RGD 
Behcet's disease  ISOMIR155 (Homo sapiens)25671481; 25671481 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Mir155  (microRNA 155)

Genes (Mus musculus)
Mir155  (microRNA 155)

Genes (Homo sapiens)
MIR155  (microRNA 155)

Additional Information