RGD Reference Report - Increased expression of ADAM 9 and ADAM 15 mRNA in pancreatic cancer. - Rat Genome Database

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Increased expression of ADAM 9 and ADAM 15 mRNA in pancreatic cancer.

Authors: Yamada, D  Ohuchida, K  Mizumoto, K  Ohhashi, S  Yu, J  Egami, T  Fujita, H  Nagai, E  Tanaka, M 
Citation: Yamada D, etal., Anticancer Res. 2007 Mar-Apr;27(2):793-9.
RGD ID: 2325247
Pubmed: PMID:17465204   (View Abstract at PubMed)

BACKGROUND: A disintegrin and metalloproteases (ADAMs) comprise a multifunctional family of membrane-anchored proteins. ADAM 9 and ADAM 15 are involved in cell migration and invasion. Expression of ADAM 9 and ADAM 15 was reported to be altered in several types of cancer. MATERIALS AND METHODS: Quantitative real-time reverse transcription-polymerase chain reaction was performed to measure the expression of ADAM 9 mRNA in bulk pancreatic tissues. Results showed no significant difference in the expression of ADAM 9 mRNA between pancreatic cancer and non-neoplastic pancreas. Primary cultured pancreatic fibroblasts also expressed ADAM 9 mRNA. Therefore, a laser microdissection and pressure catapulting technique was employed to isolate cancer cells from tumor tissues. The expression of ADAM 9 and ADAM 15 mRNA was measured in microdissected samples (cancer cells, n = 11; normal epithelial cells, n = 13 for ADAM 9; cancer cells, n = 9; normal epithelial cells, n = 9 for ADAM 15). RESULTS: Pancreatic cancer cells expressed significantly higher levels of ADAM 9 and ADAM 15 mRNA than did normal pancreatic epithelial cells (p = 0.016 for ADAM 9; p = 0.004 for ADAM 15). CONCLUSION: ADAM 9 and ADAM 15 are involved in pancreatic cancer. Microdissection-based analysis appears to be indispensable for the accurate analysis of the expression of certain ADAM family members in pancreatic cancer.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
ADAM15Humanpancreatic cancer  IEP mRNA:increased expression:pancreas and epithelial cellRGD 
ADAM9Humanpancreatic cancer  IEP mRNA:increased expression:pancreas and epithelial cellRGD 
Adam15Ratpancreatic cancer  ISOADAM15 (Homo sapiens)mRNA:increased expression:pancreas and epithelial cellRGD 
Adam15Mousepancreatic cancer  ISOADAM15 (Homo sapiens)mRNA:increased expression:pancreas and epithelial cellRGD 
Adam9Ratpancreatic cancer  ISOADAM9 (Homo sapiens)mRNA:increased expression:pancreas and epithelial cellRGD 
Adam9Mousepancreatic cancer  ISOADAM9 (Homo sapiens)mRNA:increased expression:pancreas and epithelial cellRGD 

Objects Annotated

Genes (Rattus norvegicus)
Adam15  (ADAM metallopeptidase domain 15)
Adam9  (ADAM metallopeptidase domain 9)

Genes (Mus musculus)
Adam15  (ADAM metallopeptidase domain 15)
Adam9  (ADAM metallopeptidase domain 9)

Genes (Homo sapiens)
ADAM15  (ADAM metallopeptidase domain 15)
ADAM9  (ADAM metallopeptidase domain 9)


Additional Information