RGD Reference Report - Altered mucin gene expression in stone-containing intrahepatic bile ducts and cholangiocarcinomas. - Rat Genome Database

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Altered mucin gene expression in stone-containing intrahepatic bile ducts and cholangiocarcinomas.

Authors: Lee, KT  Liu, TS 
Citation: Lee KT and Liu TS, Dig Dis Sci. 2001 Oct;46(10):2166-72.
RGD ID: 2324948
Pubmed: PMID:11680592   (View Abstract at PubMed)

Neoplastic transformation of epithelial cells is commonly associated with alterations in the synthesis and structures of mucin. Mucin protein epitopes and mRNA levels were frequently altered in adenocarcinomas compared to corresponding normal tissues. Clinically, hepatolithiasis has been regarded as a risk factor for cholangiocarcinoma. The aims of this study were to determine the possible alteration of mucin gene expression in stone-containing intrahepatic bile ducts and cholangiocarcinomas and to try to predict whether or not hepatolithiasis has a predisposition to development of cholangiocarcinoma. In situ hybridization with DIG-tailed oligonucleotides was performed on sections of paraffin-embedded tissues of stone-containing intrahepatic bile ducts, cholangiocarcinomas, and normal controls to identify the expression of MUC2, MUC3, MUC4, MUC5B, and MUC5AC in nonneoplastic and neoplastic biliary epithelium. The findings showed that (1) while multiple diverse mucin genes were expressed in the biliary epithelium, MUC3 and MUC5B mRNA were the main mucin genes expressed in the biliary epithelium of stone-containing intrahepatic bile ducts and normal controls; (2) absent or decreased expression of MUC2, MUC3, and MUC5B of mRNA was found in cholangiocarcinomas in contrast to nonneoplastic biliary epithelium; and (3) increased expression of MUC4 and MU5AC of mRNA was found in cholangiocarcinomas and the biliary epithelium, especially for dysplastic cells of stone-containing intrahepatic bile ducts compared with normal controls. In this study, using in situ hybridization we demonstrated that neoplastic transformation of the biliary epithelium is accompained by alterations in mucin gene expression, the altered mucin gene expression in dysplastic cells of stone-containing intrahepatic bile ducts may reflect a higher potential for malignant transformation in these cells, and it could be a precursor of cholangiocarcinoma in the presence of hepatolithiasis.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
MUC2Humancholangiocarcinoma  IEP mRNA:decreased expression:bile ductRGD 
MUC3AHumancholangiocarcinoma  IEP mRNA:decreased expression:bile ductRGD 
MUC5ACHumancholangiocarcinoma  IEP mRNA:increased expression:bile ductRGD 
MUC5BHumancholangiocarcinoma  IEP mRNA:decreased expression:bile ductRGD 
Muc17Ratcholangiocarcinoma  ISOMUC3A (Homo sapiens)mRNA:decreased expression:bile ductRGD 
Muc2Ratcholangiocarcinoma  ISOMUC2 (Homo sapiens)mRNA:decreased expression:bile ductRGD 
Muc2Mousecholangiocarcinoma  ISOMUC2 (Homo sapiens)mRNA:decreased expression:bile ductRGD 
Muc5acRatcholangiocarcinoma  ISOMUC5AC (Homo sapiens)mRNA:increased expression:bile ductRGD 
Muc5acMousecholangiocarcinoma  ISOMUC5AC (Homo sapiens)mRNA:increased expression:bile ductRGD 
Muc5bRatcholangiocarcinoma  ISOMUC5B (Homo sapiens)mRNA:decreased expression:bile ductRGD 
Muc5bMousecholangiocarcinoma  ISOMUC5B (Homo sapiens)mRNA:decreased expression:bile ductRGD 

Objects Annotated

Genes (Rattus norvegicus)
Muc17  (mucin 17, cell surface associated)
Muc2  (mucin 2, oligomeric mucus/gel-forming)
Muc5ac  (mucin 5AC, oligomeric mucus/gel-forming)
Muc5b  (mucin 5B, oligomeric mucus/gel-forming)

Genes (Mus musculus)
Muc2  (mucin 2)
Muc5ac  (mucin 5, subtypes A and C, tracheobronchial/gastric)
Muc5b  (mucin 5, subtype B, tracheobronchial)

Genes (Homo sapiens)
MUC2  (mucin 2, oligomeric mucus/gel-forming)
MUC3A  (mucin 3A, cell surface associated)
MUC5AC  (mucin 5AC, oligomeric mucus/gel-forming)
MUC5B  (mucin 5B, oligomeric mucus/gel-forming)


Additional Information