RGD Reference Report - Gene expression profiling identifies markers of ampullary adenocarcinoma. - Rat Genome Database

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Gene expression profiling identifies markers of ampullary adenocarcinoma.

Authors: Van Heek, NT  Maitra, A  Koopmann, J  Fedarko, N  Jain, A  Rahman, A  Iacobuzio-Donahue, CA  Adsay, V  Ashfaq, R  Yeo, CJ  Cameron, JL  Offerhaus, JA  Hruban, RH  Berg, KD  Goggins, M 
Citation: Van Heek NT, etal., Cancer Biol Ther. 2004 Jul;3(7):651-6. Epub 2004 Jul 9.
RGD ID: 2317789
Pubmed: PMID:15136764   (View Abstract at PubMed)

Ampullary adenocarcinoma is an aggressive cancer with a poor prognosis. Without surgical resection, ampullary adenocarcinomas can be difficult to distinguish from ampullary adenomas. The aim of this study was to identify differentially expressed genes in ampullary adenocarcinoma in order to identify candidate markers of the disease. The Affymetrix Human Genome U133 GeneChip set (HG-U133A and HG-U133B) was used to obtain gene expression profiles of 5 ampullary adenocarcinomas and 10 normal duodenal samples. Using fold change analysis we identified 235 fragments expressed at least fivefold higher in ampullary cancers than in normal duodenum. The expression profiles of eight candidate overexpressed genes (osteopontin, mesothelin, tissue inhibitor of metalloproteinases 1, mucin-1, mucin-5, fascin, heat shock protein 47, fibronectin 1) were confirmed by immunohistochemistry or in situ hybridization on tissue microarrays (TMA) containing 54 ampullary adenocarcinomas. One of these genes, osteopontin, was expressed 27-fold higher levels in ampullary adenocarcinomas compared to normal duodenum by genechip analysis. We therefore determined serum osteopontin levels in patients with ampullary neoplasms, patients with other periampullary diseases and in normal controls. Mean preoperative serum osteopontin levels as measured by competitive ELISA were 906 +/- 268 ng/ml in patients with ampullary cancer, 867 +/- 160 ng/ml in patients with an ampullary adenoma, 327.1 +/- 195.6 ng/ml in patients with nonmalignant periampullary diseases and 204 +/- 65 ng/ml in age-matched healthy controls (p < 0.001). Measurement of markers of ampullary cancer such as osteopontin may aid in the early detection and differential diagnosis of patients with periampullary lesions.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
FSCN1Humancommon bile duct neoplasm  IEP mRNA and protein:increased expression: :RGD 
Fscn1Ratcommon bile duct neoplasm  ISOFSCN1 (Homo sapiens)mRNA and protein:increased expression: :RGD 
Fscn1Mousecommon bile duct neoplasm  ISOFSCN1 (Homo sapiens)mRNA and protein:increased expression: :RGD 

Objects Annotated

Genes (Rattus norvegicus)
Fscn1  (fascin actin-bundling protein 1)

Genes (Mus musculus)
Fscn1  (fascin actin-bundling protein 1)

Genes (Homo sapiens)
FSCN1  (fascin actin-bundling protein 1)


Additional Information