RGD Reference Report - Localization of proinsulin and insulin in human insulinoma: preliminary immunohistochemical results. - Rat Genome Database

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Localization of proinsulin and insulin in human insulinoma: preliminary immunohistochemical results.

Authors: Roth, J  Kasper, M  Stamm, B  Hacki, WH  Storch, MJ  Madsen, OD  Kloppel, G  Heitz, PU 
Citation: Roth J, etal., Virchows Arch B Cell Pathol Incl Mol Pathol. 1989;56(5):287-92.
RGD ID: 2317268
Pubmed: PMID:2565624   (View Abstract at PubMed)

We have carried out an immunohistochemical investigation of 15 human insulinomas applying monoclonal antibodies specifically recognizing proinsulin and insulin. Our results demonstrate that the epitopes unique to proinsulin and insulin can be detected with the respective monoclonal antibodies using the protein A-gold technique after routine formaldehyde fixation and paraffin embedding of the tissues. The immunostaining pattern for proinsulin and insulin in the insulinomas was different from the observed in B cells of pancreatic islets present in the adjacent normal pancreas. Furthermore, the pattern of immunostaining was found to vary from tumor to tumor. These findings strongly suggest the possibility of a disturbed proinsulin to insulin conversion in human insulinomas.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
INSHumaninsulinoma  IDA protein:altered localization:pancreasRGD 
Ins2Ratinsulinoma  ISORGD:731056protein:altered localization:pancreasRGD 
Ins2Mouseinsulinoma  ISORGD:731056protein:altered localization:pancreasRGD 

Objects Annotated

Genes (Rattus norvegicus)
Ins2  (insulin 2)

Genes (Mus musculus)
Ins2  (insulin II)

Genes (Homo sapiens)
INS  (insulin)


Additional Information