RGD Reference Report - Management of erectile function by penile purinergic p2 receptors in the diabetic rat. - Rat Genome Database

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Management of erectile function by penile purinergic p2 receptors in the diabetic rat.

Authors: Gur, S  Kadowitz, PJ  Abdel-Mageed, AB  Kendirci, M  Sikka, SC  Burnstock, G  Hellstrom, WJ 
Citation: Gur S, etal., J Urol. 2009 May;181(5):2375-82. Epub 2009 Mar 19.
RGD ID: 2315809
Pubmed: PMID:19303093   (View Abstract at PubMed)
DOI: DOI:10.1016/j.juro.2009.01.002   (Journal Full-text)

PURPOSE: We determined the role of purine and pyrimidine nucleotides in erectile function in diabetic rats. MATERIALS AND METHODS: A total of 60 adult male rats were divided into 2 groups, including 30 controls and 30 treated with streptozotocin (60 mg/kg) for 8 weeks to induce hyperglycemia. Changes in intracavernous pressure after intracrural injections of adenosine 5'triphosphate and adenosine 5'triphosphate analogues in control and diabetic rats, and the relaxant response to electrical field stimulation of precontracted corpus cavernosum smooth muscle in organ baths were investigated. The localization of P2X1, P2Y1 and P2Y2 receptors was assessed in penile tissue via an immunohistochemical approach. RESULTS: Corpus cavernosum smooth muscle relaxation in vivo and by electrical field stimulation in vitro was significantly decreased in diabetic rats. Adenosine 5'triphosphate (P2X, P2Y), 2-methylthioadenosine 5'triphosphate (P2Y1) and uridine 5'-triphosphate (P2Y2) agonists but not alpha,beta-methylene adenosine 5'triphosphate (a P2X1 agonist) significantly improved the erectile response to electrical field stimulation in diabetic rat corpus cavernosum smooth muscle. Although intracavernous pressure/mean arterial pressure values in the rats were not restored in the presence of the P2X1 antagonist PPADS, the relaxation response to electrical field stimulation in isolated corpus cavernosum smooth muscle from diabetic rats was improved. Abundant immunoreactivity for PX1 and P2Y2 receptors was observed in penile tissues from diabetic rats compared to that from control rats. CONCLUSIONS: These results demonstrate 1) heterogeneous effects of purinergic agonists on corporeal function in diabetic rats, and 2) the activation of P2Y1 and P2Y2 receptor relaxation of corpus cavernosum smooth muscle to induce erection in rats and perhaps improve erectile function in men with diabetes.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
P2RY1Humanimpotence  ISOP2ry1 (Rattus norvegicus)associated with Diabetes Mellitus and ExperimentalRGD 
P2RY2Humanimpotence  ISOP2ry2 (Rattus norvegicus)associated with Diabetes Mellitus and ExperimentalRGD 
P2ry1Ratimpotence  IDA associated with Diabetes Mellitus and ExperimentalRGD 
P2ry1Mouseimpotence  ISOP2ry1 (Rattus norvegicus)associated with Diabetes Mellitus and ExperimentalRGD 
P2ry2Ratimpotence  IDA associated with Diabetes Mellitus and ExperimentalRGD 
P2ry2Mouseimpotence  ISOP2ry2 (Rattus norvegicus)associated with Diabetes Mellitus and ExperimentalRGD 

Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
P2ry1Ratpositive regulation of penile erection  IDA  RGD 
P2ry2Ratpositive regulation of penile erection  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
P2ry1  (purinergic receptor P2Y1)
P2ry2  (purinergic receptor P2Y2)

Genes (Mus musculus)
P2ry1  (purinergic receptor P2Y, G-protein coupled 1)
P2ry2  (purinergic receptor P2Y, G-protein coupled 2)

Genes (Homo sapiens)
P2RY1  (purinergic receptor P2Y1)
P2RY2  (purinergic receptor P2Y2)


Additional Information