RGD Reference Report - Ocular alcohol dehydrogenase in the rat: regional distribution and kinetics of the ADH-1 isoenzyme with retinol and retinal. - Rat Genome Database

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Ocular alcohol dehydrogenase in the rat: regional distribution and kinetics of the ADH-1 isoenzyme with retinol and retinal.

Authors: Julia, P  Farres, J  Pares, X 
Citation: Julia P, etal., Exp Eye Res. 1986 Apr;42(4):305-14.
RGD ID: 2315740
Pubmed: PMID:2940107   (View Abstract at PubMed)

Starch-gel electrophoresis of rat ocular tissues shows two anodic isoenzymes of alcohol dehydrogenase (ADH), designated as ADH-1 and ADH-2, ADH-1 is characteristic of the ocular tissues, and corresponds to more than 95% of all ADH activity in the eye. The well known cathodic forms of rat liver ADH, that we named ADH-3, are not observed in the ocular tissues. ADH-1 is detected in retina, pigment epithelium-choroid, ocular fluid, and cornea but not in the lens. The cornea exhibits the highest ADH activity [200 +/- 59 milliunits (munits) mg-1] followed by the pigment epithelium-choroid (11 +/- 7 munits mg-1). Activity in the retina is very small (0.6 +/- 0.2 munit mg-1) and represents only 0.6% of the total activity in the eye. Most of the rat ocular ADH is localized in the cornea (68%) where it could play a significant role in the detoxication of the alcohols of a broad range of structures. Purified ADH-1 shows a low Km for retinol oxidation (20 microM) and for retinal reduction (30 microM) indicating that this isoenzyme may have a function in the metabolism of retinoids. Ethanol competitively inhibits retinol oxidation, but with a very high apparent inhibition constant (0.6 M) demonstrating that the inhibitory effect is not significantly at the usual concentrations found in the blood during ethanol intoxication.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Adh1cRatall-trans-retinol dehydrogenase (NAD+) activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Adh1c  (alcohol dehydrogenase 1C (class I), gamma polypeptide)


Additional Information