RGD Reference Report - DNA topoisomerase I and IIalpha expression in penile carcinomas: assessing potential tumour chemosensitivity. - Rat Genome Database

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DNA topoisomerase I and IIalpha expression in penile carcinomas: assessing potential tumour chemosensitivity.

Authors: Berney, DM  Stankiewicz, E  Adlan, AM  Kudahetti, S  Biedrzycki, OJ  Hadway, P  Watkin, N  Corbishley, C 
Citation: Berney DM, etal., BJU Int. 2008 Sep;102(8):1040-4. Epub 2008 May 16.
RGD ID: 2315128
Pubmed: PMID:18489530   (View Abstract at PubMed)
DOI: DOI:10.1111/j.1464-410X.2008.07698.x   (Journal Full-text)

OBJECTIVE: To examine the tissue expression of DNA topoisomerase I (Topo I) and IIalpha (Topo II), to pursue the possibility of future chemotherapy regimens for squamous cell carcinoma of the penis (SCCP), as high expression of Topo I might indicate sensitivity to the camptothecins, whereas high Topo II might indicate sensitivity to etoposide. PATIENTS AND METHODS: In all, 73 patients with SCCP were reviewed and then tissue samples microarrayed. These were then stained with immunohistochemistry for Topo I, Topo II and Ki-67. Tumour stage, grade and type were available. RESULTS: Topo II showed a strong positive correlation with the proliferation index as measured by Ki-67 (P < 0.001) but no correlation with Topo I. There were also strong correlations between tumour grade and Ki-67, and Topo II expression (both P < 0.001). Tumour type was also strongly correlated with Topo II and Ki-67 expression, with the highest expression in basaloid carcinomas and the lowest in verrucous carcinomas. However, Topo I expression was not correlated with any other tumour variable. CONCLUSION: The expression of Topo I is grade- and type-independent, and chemotherapy using the camptothecins is unlikely to be effective. The strong positivity of Topo II in high-grade and basaloid SCCPs suggests that treatment with etoposide or other Topo II 'poisons' might be a better target for future clinical trials.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
TOP2AHumanpenile benign neoplasm severityIEP  RGD 
Top2aRatpenile benign neoplasm severityISOTOP2A (Homo sapiens) RGD 
Top2aMousepenile benign neoplasm severityISOTOP2A (Homo sapiens) RGD 

Objects Annotated

Genes (Rattus norvegicus)
Top2a  (DNA topoisomerase II alpha)

Genes (Mus musculus)
Top2a  (topoisomerase (DNA) II alpha)

Genes (Homo sapiens)
TOP2A  (DNA topoisomerase II alpha)


Additional Information