RGD Reference Report - Renal expression of exchange protein directly activated by cAMP (Epac) 1 and 2. - Rat Genome Database

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Renal expression of exchange protein directly activated by cAMP (Epac) 1 and 2.

Authors: Li, Y  Konings, IB  Zhao, J  Price, LS  De Heer, E  Deen, PM 
Citation: Li Y, etal., Am J Physiol Renal Physiol. 2008 Aug;295(2):F525-33. Epub 2008 May 21.
RGD ID: 2314762
Pubmed: PMID:18495799   (View Abstract at PubMed)
DOI: DOI:10.1152/ajprenal.00448.2007   (Journal Full-text)

In the kidney, many physiological processes of ion transport and cellular proliferation are mediated via cAMP, which classically activates protein kinase A (PKA). Recently, however, two new cAMP targets, the exchange protein directly activated by cAMP (Epac) 1 and 2, were identified, which mediate alternative pathways to PKA. To investigate their renal expression, antibodies specifically recognizing Epac1 and Epac2 were generated and used in rat immunohistochemistry with antibodies recognizing aquaporin-1 (AQP1), Tamm-Horsfall protein, Calbindin-D(28K), and AQP2 to mark proximal tubules (PT)/thin descending limbs of Henle's loop (tDLH), thick ascending limbs of Henle's loop (TAL), distal convoluted tubule/connecting tubule (DCT/CNT), and the collecting duct (CD) principal cells, respectively. Epac1 and Epac2 were expressed at the brush border of PT cells but were absent from tDLH cells. In the TAL, Epac1 and Epac2 were expressed throughout the cells with some confinement toward the apical membrane. In the DCT/CNT, Epac1 was confined to the apical region of the cells, whereas Epac2 was mainly expressed in the apical and basolateral regions. In the CD, a dispersed Epac1 expression was found in intercalated cells only (cortical CD), principal and intercalated cells [outer medullary CD (OMCD)], and mainly AQP2-negative cells in the inner medullary CD (IMCD). In contrast, Epac2 expression was at the apical and basolateral membrane of cortical principal cells, dispersed and apical in the OMCD, and in all cells of the IMCD. A similar distribution for Epac1/2 was found in the human kidney. The observed expression in different tubular segments suggests a major role for Epac 1/2 in tubular transport physiology and cellular proliferation.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Rapgef3Ratapical part of cell  IDA  RGD 
Rapgef4Ratapical plasma membrane  IDA  RGD 
Rapgef3Ratbasolateral part of cell  IDA  RGD 
Rapgef4Ratbasolateral plasma membrane  IDA  RGD 
Rapgef4Ratbrush border  IDA  RGD 
Rapgef3Ratbrush border membrane  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Rapgef3  (Rap guanine nucleotide exchange factor 3)
Rapgef4  (Rap guanine nucleotide exchange factor 4)


Additional Information