RGD Reference Report - Involvement of eukaryotic translation initiation factor 5A (eIF5A) in skeletal muscle stem cell differentiation. - Rat Genome Database

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Involvement of eukaryotic translation initiation factor 5A (eIF5A) in skeletal muscle stem cell differentiation.

Authors: Luchessi, AD  Cambiaghi, TD  Hirabara, SM  Lambertucci, RH  Silveira, LR  Baptista, IL  Moriscot, AS  Costa-Neto, CM  Curi, R 
Citation: Luchessi AD, etal., J Cell Physiol. 2009 Mar;218(3):480-9.
RGD ID: 2308859
Pubmed: PMID:19006180   (View Abstract at PubMed)
DOI: DOI:10.1002/jcp.21619   (Journal Full-text)

The eukaryotic translation initiation factor 5A (eIF5A) contains a special amino acid residue named hypusine that is required for its activity, being produced by a post-translational modification using spermidine as substrate. Stem cells from rat skeletal muscles (satellite cells) were submitted to differentiation and an increase of eIF5A gene expression was observed. Higher content of eIF5A protein was found in satellite cells on differentiation in comparison to non-differentiated satellite cells and skeletal muscle. The treatment with N1-guanyl-1,7-diaminoheptane (GC7), a hypusination inhibitor, reversibly abolished the differentiation process. In association with the differentiation blockage, an increase of glucose consumption and lactate production and a decrease of glucose and palmitic acid oxidation were observed. A reduction in cell proliferation and protein synthesis was also observed. L-Arginine, a spermidine precursor and partial suppressor of muscle dystrophic phenotype, partially abolished the GC7 inhibitory effect on satellite cell differentiation. These results reveal a new physiological role for eIF5A and contribute to elucidate the molecular mechanisms involved in muscle regeneration.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Eif5aRatpositive regulation of muscle cell differentiation  IMP  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Eif5a  (eukaryotic translation initiation factor 5A)


Additional Information