RGD Reference Report - Annexin A2 interactions with Rab14 in alveolar type II cells. - Rat Genome Database

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Annexin A2 interactions with Rab14 in alveolar type II cells.

Authors: Gou, D  Mishra, A  Weng, T  Su, L  Chintagari, NR  Wang, Z  Zhang, H  Gao, L  Wang, P  Stricker, HM  Liu, L 
Citation: Gou D, etal., J Biol Chem. 2008 May 9;283(19):13156-64. Epub 2008 Mar 10.
RGD ID: 2306298
Pubmed: PMID:18332131   (View Abstract at PubMed)
PMCID: PMC2442313   (View Article at PubMed Central)
DOI: DOI:10.1074/jbc.M801532200   (Journal Full-text)

Annexin A2, a calcium-dependent phospholipid-binding protein, is abundantly expressed in alveolar type II cells where it plays a role in lung surfactant secretion. Nevertheless, little is known about the details of its cellular pathways. To identify annexin A2-regulated or associated proteins, we silenced endogenous annexin A2 expression in rat alveolar type II cells by RNA interference and assessed the change of the cellular transcriptome by DNA microarray analysis. The loss of annexin A2 resulted in the change of 61 genes. Thirteen of the selected genes (11 down-regulated and 2 up-regulated genes) were validated by real time quantitative PCR. When the loss of rat annexin A2 was rescued by overexpressing EGFP-tagged human annexin A2, six of seven selected targets returned to their normal expression level, indicating that these genes are indeed annexin A2-associated targets. One of the targets, Rab14, co-immunoprecipitated with annexin A2. Rab14 also co-localized in part with annexin A2 and lamellar bodies in alveolar type II cells. The silencing of Rab14 resulted in a decrease in surfactant secretion, suggesting that Rab14 may play a role in surfactant secretion.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Anxa2Ratbody fluid secretion  IMP  RGD 
Rab14Ratbody fluid secretion  IMP  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Rab14Ratalveolar lamellar body  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Rab14Ratprotein binding  IPIRGD:621170 RGD 
Anxa2Ratsmall GTPase binding  IPIRGD:620881 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Anxa2  (annexin A2)
Rab14  (RAB14, member RAS oncogene family)


Additional Information