RGD Reference Report - Targeting signals and subunit interactions in coated vesicle adaptor complexes. - Rat Genome Database

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Targeting signals and subunit interactions in coated vesicle adaptor complexes.

Authors: Page, LJ  Robinson, MS 
Citation: Page LJ and Robinson MS, J Cell Biol. 1995 Nov;131(3):619-30.
RGD ID: 2306270
Pubmed: PMID:7593184   (View Abstract at PubMed)
PMCID: PMC2120623   (View Article at PubMed Central)

There are two clathrin-coated vesicle adaptor complexes in the cell, one associated with the plasma membrane and one associated with the TGN. The subunit composition of the plasma membrane adaptor complex is alpha-adaptin, beta-adaptin, AP50, and AP17; while that of the TGN adaptor complex is gamma-adaptin, beta'-adaptin, AP47, and AP19. To search for adaptor targeting signals, we have constructed chimeras between alpha-adaptin and gamma-adaptin within their NH2-terminal domains. We have identified stretches of sequence in the two proteins between amino acids approximately 130 and 330-350 that are essential for targeting. Immunoprecipitation reveals that this region determines whether a construct coassemblies with AP50 and AP17, or with AP47 and AP19. These observations suggest that these other subunits may play an important role in targeting. In contrast, beta- and beta'-adaptins are clearly not involved in this event. Chimeras between the alpha- and gamma-adaptin COOH-terminal domains reveal the presence of a second targeting signal. We have further investigated the interactions between the adaptor subunits using the yeast two-hybrid system. Interactions can be detected between the beta/beta'-adaptins and the alpha/gamma-adaptins, between the beta/beta'-adaptins and the AP50/AP47 subunits, between alpha-adaptin and AP17, and between gamma-adaptin and AP19. These results indicate that the adaptor subunits act in concert to target the complex to the appropriate membrane.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Ap2s1RatAP-2 adaptor complex  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Ap2s1  (adaptor related protein complex 2 subunit sigma 1)

Objects referenced in this article
Gene Ap2b1 adaptor related protein complex 2 subunit beta 1 Rattus norvegicus

Additional Information