RGD Reference Report - Translocation of the glucose transporter GLUT4 in cardiac myocytes of the rat. - Rat Genome Database

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Translocation of the glucose transporter GLUT4 in cardiac myocytes of the rat.

Authors: Slot, JW  Geuze, HJ  Gigengack, S  James, DE  Lienhard, GE 
Citation: Slot JW, etal., Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7815-9.
RGD ID: 2306204
Pubmed: PMID:1881917   (View Abstract at PubMed)
PMCID: PMC52394   (View Article at PubMed Central)

The insulin-regulated glucose transporter GLUT4 was immunolocalized in rat cardiac muscle under conditions of basal and stimulated glucose uptake, achieved by fasting and a combined exercise/insulin stimulus, respectively. In basal myocytes there was very little (less than 1%) GLUT4 in the different domains of the plasma membrane (sarcolemma, intercalated disk, and transverse tubular system). GLUT4 was localized in small tubulo-vesicular elements that occur predominantly near the sarcolemma and the transverse tubular system and in the trans-Golgi region. Upon stimulation approximately 42% of GLUT4 was found in the plasma membrane. Each domain of the plasma membrane contributed equally to this effect. GLUT4-positive, clathrin-coated pits were also present at each cell surface domain. The remainder of the labeling was in tubulo-vesicular elements at the same sites as in basal cells and in the intercalated disk areas. The localization of GLUT4 in cardiac myocytes is essentially the same as in brown adipocytes, skeletal muscle, and white adipocytes. We conclude that increased glucose transport in muscle and fat is accounted for by translocation of GLUT4 from the intracellular tubulo-vesicular elements to the plasma membrane. The labeling of coated pits indicates that in stimulated myocytes, as in adipocytes, GLUT4 recycles constantly between the endosomal compartment and the plasma membrane and that stimulation of the exocytotic rate constant is likely the major mechanism for GLUT4 translocation.



Gene Ontology Annotations    Click to see Annotation Detail View

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc2a4Ratclathrin-coated pit  IDA  RGD 
Slc2a4Ratmultivesicular body  IDA  RGD 
Slc2a4Ratsarcolemma  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc2a4  (solute carrier family 2 member 4)


Additional Information