RGD Reference Report - Identification of heterogeneous nuclear ribonucleoprotein K (hnRNP K) as a repressor of C/EBPbeta-mediated gene activation. - Rat Genome Database

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Identification of heterogeneous nuclear ribonucleoprotein K (hnRNP K) as a repressor of C/EBPbeta-mediated gene activation.

Authors: Miau, LH  Chang, CJ  Shen, BJ  Tsai, WH  Lee, SC 
Citation: Miau LH, etal., J Biol Chem. 1998 Apr 24;273(17):10784-91.
RGD ID: 2303817
Pubmed: PMID:9553145   (View Abstract at PubMed)

Transcription factor C/EBPbeta has been known to regulate a wide array of genes including those involved in the acute-phase response. One of the molecular mechanisms underlying transcription activation by C/EBPbeta is through protein-protein interaction with other transcription factors. Here we report the identification and characterization of physical and functional interactions between C/EBPbeta and heterogeneous nuclear ribonucleoprotein (hnRNP) K. This interaction results in the repression of C/EBPbeta-dependent trans-activation of the agp gene. Footprinting assays indicate that hnRNP K cannot bind to the promoter region of agp gene or interfere with the binding of C/EBPbeta to its cognate DNA site. Furthermore, agp gene activation by the synergistic interaction of Nopp140 and C/EBPbeta is abolished by hnRNP K. The kinetics of appearance of C/EBPbeta-hnRNP K complex in the nuclear extract after initiation of acute-phase reaction indicates that hnRNP K functions as a negative regulator of C/EBPbeta-mediated activation of agp gene.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
HnrnpkRatacute-phase response  IEP  RGD 
HnrnpkRatnegative regulation of gene expression  IMP Orm1RGD 
HnrnpkRatnegative regulation of protein binding  IMP  RGD 
Nolc1Ratpositive regulation of DNA-templated transcription  IDA  RGD 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
HnrnpkRatprotein-DNA complex  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CebpbRatprotein binding  IPIRGD:621578 RGD 
CebpbRatprotein binding  IPIRGD:71058 RGD 
HnrnpkRatprotein domain specific binding  IPIRGD:2327 RGD 
Nolc1Ratprotein domain specific binding  IPIRGD:2327 RGD 
HnrnpkRatribonucleoprotein complex binding  IDA  RGD 
HnrnpkRattranscription factor binding  IPIRGD:2327 RGD 
Nolc1Rattranscription factor binding  IPIRGD:2327 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Cebpb  (CCAAT/enhancer binding protein beta)
Hnrnpk  (heterogeneous nuclear ribonucleoprotein K)
Nolc1  (nucleolar and coiled-body phosphoprotein 1)


Additional Information