RGD Reference Report - Kinetic studies of the reaction mechanism of rat liver phosphoglycerate kinase in the direction of ADP utilization. - Rat Genome Database

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Kinetic studies of the reaction mechanism of rat liver phosphoglycerate kinase in the direction of ADP utilization.

Authors: Lavoinne, A  Marchand, JC  Chedeville, A  Matray, F 
Citation: Lavoinne A, etal., Biochimie. 1983 Mar;65(3):211-20.
RGD ID: 2302859
Pubmed: PMID:6405813   (View Abstract at PubMed)

The kinetic properties of rat liver phosphoglycerate kinase were investigated in the forward direction of the reaction (utilization of ADP). The kinetic studies were performed in an assay system using combined hexokinase/glucose-6-phosphate dehydrogenase as an ATP trap. The Km values for Mg ADP1- and 1,3-diphospho-D-glycerate were approximately 0.11 and 0.006 mM, respectively. Reciprocal plots of 1/v versus 1/ (Mg ADP1-) at different fixed concentrations of 1,3-diphospho-D-glycerate and 1/v versus 1/ (1,3-diphospho-D-glycerate) at different fixed concentrations of Mg ADP1- were apparently parallel. However, product inhibition studies (3-phospho-D-glycerate), dead-end inhibition studies (2,3-diphospho-D-glycerate), and adenosine and AMP inhibition patterns yielded results consistent with a rapid equilibrium random mechanism in which the binding of one substrate greatly decreases the affinity of the enzyme for the second substrate. Existence of two sites for 3-phospho-D-glycerate is suggested.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Pgk1Ratglycolytic process  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Pgk1RatADP binding  IDA  RGD 
Pgk1Ratphosphoglycerate kinase activity  IDA  RGD 

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations


  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
PGK1Humanglycolysis pathway   ISORGD:619878 RGD 
Pgk1Mouseglycolysis pathway   ISORGD:619878 RGD 
Pgk1Ratglycolysis pathway   IDA  RGD 
Objects Annotated

Genes (Rattus norvegicus)
Pgk1  (phosphoglycerate kinase 1)

Genes (Mus musculus)
Pgk1  (phosphoglycerate kinase 1)

Genes (Homo sapiens)
PGK1  (phosphoglycerate kinase 1)


Additional Information