RGD Reference Report - Monitoring clathrin-mediated endocytosis during synaptic activity. - Rat Genome Database

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Monitoring clathrin-mediated endocytosis during synaptic activity.

Authors: Mueller, VJ  Wienisch, M  Nehring, RB  Klingauf, J 
Citation: Mueller VJ, etal., J Neurosci. 2004 Feb 25;24(8):2004-12.
RGD ID: 2301373
Pubmed: PMID:14985443   (View Abstract at PubMed)
PMCID: PMC6730391   (View Article at PubMed Central)
DOI: DOI:10.1523/JNEUROSCI.4080-03.2004   (Journal Full-text)

To visualize clathrin redistribution during endocytosis in hippocampal boutons, we used a fusion protein of clathrin light chain with enhanced green fluorescent protein. Both high potassium and electric field stimulation lead after a stimulus-dependent delay to a transient increase of fluorescence in synapses, but a slight and transient decrease in adjacent axonal segments. We conclude that the rise and fall of the signal in boutons, with decay kinetics remarkably similar to previous estimates of the endocytic time course, reflects coat assembly and disassembly. Thus, we could selectively measure clathrin-mediated endocytosis and separate its kinetics from other modes of membrane retrieval in CNS synapses. A long-lasting delay preceding the fluorescent transients shows that endocytosis during the first few seconds of continuing stimulation cannot be mediated by newly formed clathrin-coated pits. Therefore, a fast mode of endocytosis is either clathrin-independent or involves preassembled (easily retrievable) clathrin lattices at sites of endocytosis.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CltaRatendocytosis  IEP  RGD 
CltaRatsynaptic vesicle endocytosis involved_inEXP PMID:14985443SynGO 
CltaRatsynaptic vesicle endocytosis involved_inIDA PMID:14985443SynGO 
CltaRatsynaptic vesicle endocytosis involved_inIMP PMID:14985443SynGO 
CltbRatsynaptic vesicle endocytosis involved_inEXP PMID:14985443SynGO 
CltbRatsynaptic vesicle endocytosis involved_inIDA PMID:14985443SynGO 
CltbRatsynaptic vesicle endocytosis involved_inIMP PMID:14985443SynGO 
CltbRatsynaptic vesicle endocytosis involved_inNAS PMID:14985443SynGO 

Cellular Component

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
CltaRatclathrin-coated pit  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Clta  (clathrin, light chain A)
Cltb  (clathrin, light chain B)


Additional Information