RGD Reference Report - Affinity purification and characterization of serine hydroxymethyltransferases from rat liver. - Rat Genome Database

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Affinity purification and characterization of serine hydroxymethyltransferases from rat liver.

Authors: Masuda, T  Sakamoto, M  Nishizaki, I  Hayashi, H  Yamamoto, M  Wada, H 
Citation: Masuda T, etal., J Biochem. 1987 Mar;101(3):643-52.
RGD ID: 2300383
Pubmed: PMID:3110140   (View Abstract at PubMed)

A rapid and simple method was developed for the purification of serine hydroxymethyltransferases [EC 2.1.2.1]. The procedure involved ammonium sulfate precipitation, DEAE-cellulose column chromatography and affinity chromatography on an L-adsorbent. Through this procedure the cytosolic enzyme (s-SHMT) was purified 1,650-fold, and the mitochondrial enzyme (m-SHMT) 1,730-fold, with a yield of more than 30% in both cases. Both preparations gave a single band with a Mr of 54,000 on SDS-PAGE. The native enzymes both contained 4 mol of pyridoxal phosphate/mol of enzyme, and showed a Mr value of 220,000 on gel filtration, indicating a tetrameric structure. Several other properties of the enzymes were also studied.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Shmt1Ratglycine biosynthetic process  IDA from L-allothreonine in the absence of tetrahydrofolateRGD 
Shmt2Ratglycine biosynthetic process  IDA from L-allothreonine in the absence of tetrahydrofolateRGD 
Shmt1Ratglycine biosynthetic process from serine  IDA in the presence of tetrahydrofolateRGD 
Shmt2Ratglycine biosynthetic process from serine  IDA in the presence of tetrahydrofolateRGD 

Molecular Function

  

Molecular Pathway Annotations    Click to see Annotation Detail View

RGD Manual Annotations


  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SHMT1Humanfolate mediated one-carbon metabolic pathway   ISOShmt1 (Rattus norvegicus) RGD 
SHMT2Humanfolate mediated one-carbon metabolic pathway   ISOShmt2 (Rattus norvegicus) RGD 
Shmt1Mousefolate mediated one-carbon metabolic pathway   ISOShmt1 (Rattus norvegicus) RGD 
Shmt1Ratfolate mediated one-carbon metabolic pathway   IDA  RGD 
Shmt2Ratfolate mediated one-carbon metabolic pathway   IDA  RGD 
Shmt2Mousefolate mediated one-carbon metabolic pathway   ISOShmt2 (Rattus norvegicus) RGD 
SHMT1Humanglycine metabolic pathway  ISOShmt1 (Rattus norvegicus) RGD 
SHMT2Humanglycine metabolic pathway  ISOShmt2 (Rattus norvegicus) RGD 
Shmt1Mouseglycine metabolic pathway  ISOShmt1 (Rattus norvegicus) RGD 
Shmt1Ratglycine metabolic pathway  IDA  RGD 
Shmt2Ratglycine metabolic pathway  IDA  RGD 
Shmt2Mouseglycine metabolic pathway  ISOShmt2 (Rattus norvegicus) RGD 
SHMT1Humanglycine, serine and threonine metabolic pathway  ISOShmt1 (Rattus norvegicus) RGD 
SHMT2Humanglycine, serine and threonine metabolic pathway  ISOShmt2 (Rattus norvegicus) RGD 
Shmt1Mouseglycine, serine and threonine metabolic pathway  ISOShmt1 (Rattus norvegicus) RGD 
Shmt1Ratglycine, serine and threonine metabolic pathway  IDA  RGD 
Shmt2Ratglycine, serine and threonine metabolic pathway  IDA  RGD 
Shmt2Mouseglycine, serine and threonine metabolic pathway  ISOShmt2 (Rattus norvegicus) RGD 
Objects Annotated

Genes (Rattus norvegicus)
Shmt1  (serine hydroxymethyltransferase 1)
Shmt2  (serine hydroxymethyltransferase 2)

Genes (Mus musculus)
Shmt1  (serine hydroxymethyltransferase 1 (soluble))
Shmt2  (serine hydroxymethyltransferase 2 (mitochondrial))

Genes (Homo sapiens)
SHMT1  (serine hydroxymethyltransferase 1)
SHMT2  (serine hydroxymethyltransferase 2)


Additional Information