RGD Reference Report - KiSS-1 expression in human breast cancer. - Rat Genome Database

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KiSS-1 expression in human breast cancer.

Authors: Martin, TA  Watkins, G  Jiang, WG 
Citation: Martin TA, etal., Clin Exp Metastasis. 2005;22(6):503-11.
RGD ID: 2292135
Pubmed: PMID:16320113   (View Abstract at PubMed)
DOI: DOI:10.1007/s10585-005-4180-0   (Journal Full-text)

The KiSS-1 gene encodes a 145 amino acid residue peptide that is further processed to a final peptide, metastin, a ligand to a G-coupled orphan receptor (OT7T175/AXOR12). KiSS-1 has been identified as a putative human metastasis suppressor gene in melanomas and in breast cancer cell lines. This study aimed to determine the expression and distribution of KiSS-1 and its receptor in human breast cancer tissues and to identify a possible link between expression levels and patient prognosis. Frozen sections from breast cancer primary tumours (matched tumour 124 and background 33) were immuno-stained with KiSS-1 antibody. RNA was reverse transcribed and analyzed by Q-PCR (standardized using beta-actin, and normalized with cytokeratin-19 levels). Levels of expression of KiSS-1 were higher in tumour compared to background tissues (3,124+/-1,262 vs 2,397+/-1,181) and significantly increased in node positive tumours compared to node negative (3,637+/-1,719 vs 2,653+/-1,994, P = 0.02). KiSS-1 expression was also increased with increasing grade and TNM status. There were no such trends with the KiSS-1 receptor. Expression of KiSS-1 was higher in patients who had died from breast cancer than those who had remained healthy (4,631+/-3,024 vs 2,280+/-1,403) whereas expression of the receptor was reduced (480+/-162 vs 195+/-134). Immunohistochemical staining showed increased expression of KiSS-1 in tumour sections. Insertion of the KiSS-1 gene into the human breast cancer cell line MDA-MB-231, resulted in cells that were significantly more motile and invasive in behaviour, with reduced adhesion to matrix, using respective assays. In conclusion, KiSS-1 expression is increased in human breast cancer, particularly in patients with aggressive tumours and with mortality. Over-expression of KiSS-1 in breast cancer cells result in more aggressive phenotype. Together, it suggests that KiSS-1 plays a role beyond the initial metastasis repressor in this cancer type.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Breast Neoplasms disease_progressionIEP 2292135mRNA more ...RGD 
Breast Neoplasms disease_progressionISOKISS1 (Homo sapiens)2292135; 2292135 RGD 

Objects Annotated

Genes (Rattus norvegicus)
Kiss1  (KiSS-1 metastasis-suppressor)

Genes (Mus musculus)
Kiss1  (KiSS-1 metastasis-suppressor)

Genes (Homo sapiens)
KISS1  (KiSS-1 metastasis suppressor)


Additional Information