RGD Reference Report - Expression, purification, crystallization and preliminary X-ray analysis of the ligand-binding domain of metabotropic glutamate receptor 7. - Rat Genome Database

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Expression, purification, crystallization and preliminary X-ray analysis of the ligand-binding domain of metabotropic glutamate receptor 7.

Authors: Muto, T  Tsuchiya, D  Morikawa, K  Jingami, H 
Citation: Muto T, etal., Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jul 1;63(Pt 7):627-30. Epub 2007 Jun 29.
RGD ID: 2289012
Pubmed: PMID:17620729   (View Abstract at PubMed)
PMCID: PMC2335145   (View Article at PubMed Central)
DOI: DOI:10.1107/S1744309107030059   (Journal Full-text)

Glutamate is the major excitatory neurotransmitter and its metabotropic glutamate receptor (mGluR) plays an important role in the central nervous system. The ligand-binding domain (LBD) of mGluR subtype 7 (mGluR7) was produced using the baculovirus expression system and purified from the culture medium. The purified protein was characterized by gel-filtration chromatography, SDS-PAGE and a ligand-binding assay. Crystals of mGluR7 LBD were grown at 293 K by the hanging-drop vapour-diffusion method. The crystals diffracted X-rays to 3.30 A resolution using synchrotron radiation and belong to the trigonal space group P3(1)21, with unit-cell parameters a = b = 92.4, c = 114.3 A. Assuming the presence of one protomer per crystallographic asymmetric unit, the Matthews coefficient V(M) was calculated to be 2.5 A3 Da(-1) and the solvent content was 51%.



Gene Ontology Annotations    Click to see Annotation Detail View

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Grm7Ratglutamate binding  IDA  RGD 
Grm7Ratidentical protein binding  IPIGrm7 (Rattus norvegicus)homodimerizationRGD 

Objects Annotated

Genes (Rattus norvegicus)
Grm7  (glutamate metabotropic receptor 7)


Additional Information