RGD Reference Report - Determination of mir-155 and mir-146a expression rates and its association with expression level of TNF-α and CTLA4 genes in patients with Behcet's disease. - Rat Genome Database

Send us a Message



Submit Data |  Help |  Video Tutorials |  News |  Publications |  Download |  REST API |  Citing RGD |  Contact   

Determination of mir-155 and mir-146a expression rates and its association with expression level of TNF-α and CTLA4 genes in patients with Behcet's disease.

Authors: Kolahi, Sousan  Farajzadeh, Mohammad-Jahed  Alipour, Shahriar  Abhari, Alireza  Farhadi, Jafar  Bahavarnia, Neda  Malek Mahdavi, Aida  Khabbazi, Alireza  Sakhinia, Ebrahim 
Citation: Kolahi S, etal., Immunol Lett. 2018 Dec;204:55-59. doi: 10.1016/j.imlet.2018.10.012. Epub 2018 Oct 23.
RGD ID: 21409751
Pubmed: PMID:30366049   (View Abstract at PubMed)
DOI: DOI:10.1016/j.imlet.2018.10.012   (Journal Full-text)


INTRODUCTION: MicroRNAs (miRNAs) are involved in the pathogenesis of inflammatory diseases. MiR-146 and miR-155 emerged as key regulators of the immune response. This study designed to analyze the miR-146a and miR-155 expression in patients with Behcet's disease (BD) and investigated their association with the expression of tumor necrosis factor-alpha (TNF-α) and cytotoxic T lymphocyte associated antigen-4 (CTLA-4) genes.
METHODOLOGY: In a case-control study, 47 Iranian Azeri BD patients and 61 age- and sex matched healthy controls recruited to the study. Peripheral blood mononuclear cells (PBMCs) were isolated from EDTA blood tubes by Ficoll density-gradient centrifugation. Genomic DNA samples of BD and healthy controls were extracted using the rapid genomic DNA extraction method from the peripheral blood collected in tubes containing EDTA. Total RNA was extracted from the PBMCs according to the TRIzol protocol. MiR-146a, miR-155, TNF-α and CTLA-4 expression were studied using real-time PCR.
RESULTS: MiR-155 and TNF-α expression was significantly increased, whilst CTLA-4 expression was significantly decreased in the PBMCs of BD patients. There was no significant difference in the miR-146a expression rate between BD patients and controls. A positive correlation between miR-155 and TNF-α expression and negative correlation between miR-155 and CTLA-4 expression were observed. No significant association was observed between the expression of miR-155, miR-146a, TNF-α and CTLA-4 genes with BD activity. MiR-155 and miR-146a expression rate were significantly higher in patients with uveitis and phlebitis, respectively.
DISCUSSION AND CONCLUSION: The expression of miR-155 increased in BD and associated with upregulation of TNF-α and downregulation of CTLA-4 genes.

RGD Manual Disease Annotations    Click to see Annotation Detail View
TermQualifierEvidenceWithReferenceNotesSourceOriginal Reference(s)
Behcet's disease  IEP 21409751miRNA:increased expression:peripheral blood mononuclear cellRGD 
Behcet's disease  ISOMIR155 (Homo sapiens)21409751; 21409751miRNA:increased expression:peripheral blood mononuclear cellRGD 

Objects Annotated

Genes (Rattus norvegicus)
Mir155  (microRNA 155)

Genes (Mus musculus)
Mir155  (microRNA 155)

Genes (Homo sapiens)
MIR155  (microRNA 155)


Additional Information