RGD Reference Report - Expression of DPC4/Smad4 gene in stone-containing intrahepatic bile duct. - Rat Genome Database

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Expression of DPC4/Smad4 gene in stone-containing intrahepatic bile duct.

Authors: Lee, King-Teh  Chang, Wen-Tsan  Wang, Shen-Nien  Chuang, Shih-Chang  Chai, Chee-Yin  Hu, Shih-Wen 
Citation: Lee KT, etal., J Surg Oncol. 2006 Sep 15;94(4):338-43. doi: 10.1002/jso.20517.
RGD ID: 18936999
Pubmed: PMID:16917866   (View Abstract at PubMed)
DOI: DOI:10.1002/jso.20517   (Journal Full-text)


BACKGROUND AND OBJECTIVES: Hepatolithiasis is etiologically related to cholangiocarcinoma. We underwent this study with an attempt to examine the expression of DPC4/Smad4 gene in stone-containing intrahepatic bile ducts (IHD) and intrahepatic cholangiocarcinoma (ICC).
PATIENTS AND METHODS: The immunohistochemical method and RT-PCR analysis were used to study the expression of DPC4/Smad4 gene in normal IHD, stone-containing IHD, and ICC. All the specimens were from hepatic resection.
RESULTS: The immunohistochemical study showed that all specimens from 24 normal IHD had marked expression of DPC4/Smad4 gene, while there was 4.4% (2/46) and 33.3% (3/9) loss of DPC4/Smad4 expression in stone-containing IHD and ICC, respectively. Among the specimens of stone-containing IHD, all the hyperplastic epithelial cells showed normal expression of DPC4/Smad4 gene while dysplastic epithelial cells showed 20% (2/10) loss expression of DPC4/Smad4. The RT-PCR analysis showed that the normal IHD had the highest content of DPC4/Smad4 mRNA, which was threefold and sixfold higher than that of stone-containing IHD and ICC, respectively.
CONCLUSION: Loss expression of DPC4/Smad4 gene was found both in stone-containing IHD and ICC. Dysplastic epithelium of stone-containing IHD had higher potential for malignant transformation.



RGD Manual Disease Annotations    Click to see Annotation Detail View

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
SMAD4HumanGallstones  IEP mRNA and protein:decreased expression:intrahepatic bile ductRGD 
Smad4RatGallstones  ISOSMAD4 (Homo sapiens)mRNA and protein:decreased expression:intrahepatic bile ductRGD 
Smad4MouseGallstones  ISOSMAD4 (Homo sapiens)mRNA and protein:decreased expression:intrahepatic bile ductRGD 
SMAD4Humanintrahepatic cholangiocarcinoma  IEP mRNA and protein:decreased expression:intrahepatic bile ductRGD 
Smad4Ratintrahepatic cholangiocarcinoma  ISOSMAD4 (Homo sapiens)mRNA and protein:decreased expression:intrahepatic bile ductRGD 
Smad4Mouseintrahepatic cholangiocarcinoma  ISOSMAD4 (Homo sapiens)mRNA and protein:decreased expression:intrahepatic bile ductRGD 

Objects Annotated

Genes (Rattus norvegicus)
Smad4  (SMAD family member 4)

Genes (Mus musculus)
Smad4  (SMAD family member 4)

Genes (Homo sapiens)
SMAD4  (SMAD family member 4)


Additional Information