RGD Reference Report - Differential regulation of L-arginine transporters (cationic amino acid transporter-1 and -2) by peroxynitrite in rat mesangial cells. - Rat Genome Database

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Differential regulation of L-arginine transporters (cationic amino acid transporter-1 and -2) by peroxynitrite in rat mesangial cells.

Authors: Schwartz, IF  Chernichovsky, T  Hagin, D  Ingbir, M  Reshef, R  Chernin, G  Levo, Y  Schwartz, D 
Citation: Schwartz IF, etal., Nephrol Dial Transplant. 2006 Dec;21(12):3409-14. Epub 2006 Sep 23.
RGD ID: 1642934
Pubmed: PMID:16998217   (View Abstract at PubMed)
DOI: DOI:10.1093/ndt/gfl522   (Journal Full-text)

BACKGROUND: It has become evident that increased nitric oxide (NO) generation may be associated with production of reactive oxygen species, such as peroxynitrite (ONOO-). Peroxynitrite has been postulated to be responsible for several of the cytotoxic effects previously ascribed to NO. Since cellular arginine uptake has been shown to modulate nitric oxide synthase activity, we were intrigued to study the effect of ONOO- on arginine traffic in renal mesangial cells. METHODS: Arginine uptake, CAT-1 and CAT-2 mRNA expression by northern blotting analysis, and CAT-1 protein content using western blotting were determined in mesangial cells pre-treated with peroxynitrite (0.1 and 0.5 mM) for 2 h. RESULTS: Peroxynitrite induced a significant increase in arginine uptake and CAT-2 mRNA expression compared with untreated cells. In contrast, CAT-1 mRNA expression and protein abundance were diminished. CONCLUSIONS: In rat mesangial cells, peroxynitrite augments arginine uptake via augmentation of CAT-2 while decreasing CAT-1 expression.



Gene Ontology Annotations    Click to see Annotation Detail View

Biological Process

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc7a2RatL-arginine transmembrane transport  IDA  RGD 

Molecular Function

  
Object SymbolSpeciesTermQualifierEvidenceWithNotesSourceOriginal Reference(s)
Slc7a2RatL-arginine transmembrane transporter activity  IDA  RGD 

Objects Annotated

Genes (Rattus norvegicus)
Slc7a2  (solute carrier family 7 member 2)


Additional Information